Detection of the biotin-streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes

Tsouti, V; Boutopoulos, C; Andreakou, P; Ioannou, M; Zergioti, I; Goustouridis, D; Kafetzopoulos, D; Tsoukalas, D; Normand, P; Chatzandroulis, S

dc.contributor.author	Tsouti, V	en
dc.contributor.author	Boutopoulos, C	en
dc.contributor.author	Andreakou, P	en
dc.contributor.author	Ioannou, M	en
dc.contributor.author	Zergioti, I	en
dc.contributor.author	Goustouridis, D	en
dc.contributor.author	Kafetzopoulos, D	en
dc.contributor.author	Tsoukalas, D	en
dc.contributor.author	Normand, P	en
dc.contributor.author	Chatzandroulis, S	en
dc.date.accessioned	2014-03-01T01:30:09Z
dc.date.available	2014-03-01T01:30:09Z
dc.date.issued	2009	en
dc.identifier.issn	0167-9317	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19485
dc.subject	Biosensor	en
dc.subject	Biotin-streptavidin	en
dc.subject	Capacitive readout	en
dc.subject.classification	Engineering, Electrical & Electronic	en
dc.subject.classification	Nanoscience & Nanotechnology	en
dc.subject.classification	Optics	en
dc.subject.classification	Physics, Applied	en
dc.subject.other	Biological interactions	en
dc.subject.other	Biomolecular interactions	en
dc.subject.other	Biotin-streptavidin	en
dc.subject.other	Capacitance variations	en
dc.subject.other	Capacitive readout	en
dc.subject.other	Fluorescent microscopies	en
dc.subject.other	Functionalized	en
dc.subject.other	Streptavidin	en
dc.subject.other	Surface stress	en
dc.subject.other	Biosensors	en
dc.subject.other	Surface properties	en
dc.subject.other	Coenzymes	en
dc.title	Detection of the biotin-streptavidin interaction by exploiting surface stress changes on ultrathin Si membranes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.mee.2008.11.058	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.mee.2008.11.058	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	The detection of the biotin-streptavidin interaction is presented based on surface stress changes of functionalized Si membranes. During the biomolecular interaction, the surface stress of the functionalized membranes alters resulting in their deflection and finally in capacitance variations. The biological interaction on the sensors surface was verified after each experiment using fluorescent microscopy. Successful detection of 21 nM of streptavidin using 3.55 mM biotin probes is presented. (C) 2008 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Microelectronic Engineering	en
dc.identifier.doi	10.1016/j.mee.2008.11.058	en
dc.identifier.isi	ISI:000267273300275	en
dc.identifier.volume	86	en
dc.identifier.issue	4-6	en
dc.identifier.spage	1495	en
dc.identifier.epage	1498	en