DIANA-microT web server: Elucidating microRNA functions through target prediction

Maragkakis, M; Reczko, M; Simossis, VA; Alexiou, P; Papadopoulos, GL; Dalamagas, T; Giannopoulos, G; Goumas, G; Koukis, E; Kourtis, K; Vergoulis, T; Koziris, N; Sellis, T; Tsanakas, P; Hatzigeorgiou, AG

dc.contributor.author	Maragkakis, M	en
dc.contributor.author	Reczko, M	en
dc.contributor.author	Simossis, VA	en
dc.contributor.author	Alexiou, P	en
dc.contributor.author	Papadopoulos, GL	en
dc.contributor.author	Dalamagas, T	en
dc.contributor.author	Giannopoulos, G	en
dc.contributor.author	Goumas, G	en
dc.contributor.author	Koukis, E	en
dc.contributor.author	Kourtis, K	en
dc.contributor.author	Vergoulis, T	en
dc.contributor.author	Koziris, N	en
dc.contributor.author	Sellis, T	en
dc.contributor.author	Tsanakas, P	en
dc.contributor.author	Hatzigeorgiou, AG	en
dc.date.accessioned	2014-03-01T01:30:11Z
dc.date.available	2014-03-01T01:30:11Z
dc.date.issued	2009	en
dc.identifier.issn	0305-1048	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19494
dc.subject	microrna	en
dc.subject	Signal To Noise Ratio	en
dc.subject	User Interface	en
dc.subject	Kyoto Encyclopedia of Genes and Genomes	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	microRNA	en
dc.subject.other	accuracy	en
dc.subject.other	algorithm	en
dc.subject.other	article	en
dc.subject.other	client server application	en
dc.subject.other	computer interface	en
dc.subject.other	computer prediction	en
dc.subject.other	gene function	en
dc.subject.other	gene interaction	en
dc.subject.other	gene sequence	en
dc.subject.other	gene targeting	en
dc.subject.other	genetic database	en
dc.subject.other	nomenclature	en
dc.subject.other	online system	en
dc.subject.other	performance measurement system	en
dc.subject.other	priority journal	en
dc.subject.other	search engine	en
dc.subject.other	signal noise ratio	en
dc.subject.other	web browser	en
dc.subject.other	Algorithms	en
dc.subject.other	Binding Sites	en
dc.subject.other	Gene Expression Regulation	en
dc.subject.other	MicroRNAs	en
dc.subject.other	RNA, Messenger	en
dc.subject.other	Sequence Analysis, RNA	en
dc.subject.other	Software	en
dc.subject.other	User-Computer Interface	en
dc.title	DIANA-microT web server: Elucidating microRNA functions through target prediction	en
heal.type	journalArticle	en
heal.identifier.primary	10.1093/nar/gkp292	en
heal.identifier.secondary	http://dx.doi.org/10.1093/nar/gkp292	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Computational microRNA (miRNA) target prediction is one of the key means for deciphering the role of miRNAs in development and disease. Here, we present the DIANA-microT web server as the user interface to the DIANA-microT 3.0 miRNA target prediction algorithm. The web server provides extensive information for predicted miRNA: target gene interactions with a user-friendly interface, providing extensive connectivity to online biological resources. Target gene and miRNA functions may be elucidated through automated bibliographic searches and functional information is accessible through Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The web server offers links to nomenclature, sequence and protein databases, and users are facilitated by being able to search for targeted genes using different nomenclatures or functional features, such as the genes possible involvement in biological pathways. The target prediction algorithm supports parameters calculated individually for each miRNA: target gene interaction and provides a signal-to-noise ratio and a precision score that helps in the evaluation of the significance of the predicted results. Using a set of miRNA targets recently identified through the pSILAC method, the performance of several computational target prediction programs was assessed. DIANA-microT 3.0 achieved there with 66% the highest ratio of correctly predicted targets over all predicted targets. The DIANA-microT web server is freely available at www.microrna.gr/microT.	en
heal.publisher	OXFORD UNIV PRESS	en
heal.journalName	Nucleic Acids Research	en
dc.identifier.doi	10.1093/nar/gkp292	en
dc.identifier.isi	ISI:000267889100048	en
dc.identifier.volume	37	en
dc.identifier.issue	SUPPL. 2	en
dc.identifier.spage	W273	en
dc.identifier.epage	W276	en