Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system

Xiros, C; Christakopoulos, P

dc.contributor.author	Xiros, C	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:30:20Z
dc.date.available	2014-03-01T01:30:20Z
dc.date.issued	2009	en
dc.identifier.issn	1754-6834	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19558
dc.subject	Carbon Source	en
dc.subject	Enzyme	en
dc.subject	Ethanol Production	en
dc.subject	fusarium oxysporum	en
dc.subject	Growth Stage	en
dc.subject	Submerged Culture	en
dc.subject.other	Aeration rates	en
dc.subject.other	Alkali pretreatment	en
dc.subject.other	Bioconversion process	en
dc.subject.other	Bioconversion yields	en
dc.subject.other	Bioprocess	en
dc.subject.other	Biotechnological applications	en
dc.subject.other	Brewer's spent grains	en
dc.subject.other	Brewing process	en
dc.subject.other	Carbohydrate contents	en
dc.subject.other	Carbon sources	en
dc.subject.other	Cellobiohydrolase	en
dc.subject.other	Commercial applications	en
dc.subject.other	Corn cobs	en
dc.subject.other	Endo-glucanase	en
dc.subject.other	Ethanol productions	en
dc.subject.other	Ethanol yields	en
dc.subject.other	Extracellular	en
dc.subject.other	Feruloyl esterase	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Glucosidase	en
dc.subject.other	Growth stages	en
dc.subject.other	Hemicellulose hydrolysates	en
dc.subject.other	Lignocellulolytic enzymes	en
dc.subject.other	Mesophilic	en
dc.subject.other	Microaerobic conditions	en
dc.subject.other	Submerged conditions	en
dc.subject.other	Submerged cultures	en
dc.subject.other	Sugar concentrations	en
dc.subject.other	Sugar mixtures	en
dc.subject.other	Theoretical yields	en
dc.subject.other	Xylanase	en
dc.subject.other	Xylosidase	en
dc.subject.other	Biochemical engineering	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Cellulose	en
dc.subject.other	Enzymatic hydrolysis	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucose	en
dc.subject.other	Hydrolysis	en
dc.subject.other	Mixtures	en
dc.subject.other	Sugar (sucrose)	en
dc.subject.other	Ethanol	en
dc.subject.other	biotechnology	en
dc.subject.other	enzyme	en
dc.subject.other	ethanol	en
dc.subject.other	fermentation	en
dc.subject.other	Concentration	en
dc.subject.other	Conversion	en
dc.subject.other	Cost Effectiveness	en
dc.subject.other	Enzymatic Activity	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucose	en
dc.subject.other	Production Control	en
dc.subject.other	Xylose	en
dc.subject.other	Fungi	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Zea mays	en
dc.title	Enhanced ethanol production from brewer's spent grain by a Fusarium oxysporum consolidated system	en
heal.type	journalArticle	en
heal.identifier.primary	10.1186/1754-6834-2-4	en
heal.identifier.secondary	http://dx.doi.org/10.1186/1754-6834-2-4	en
heal.identifier.secondary	4	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Background: Brewer's spent grain (BG), a by-product of the brewing process, is attracting increasing scientific interest as a low-cost feedstock for many biotechnological applications. BG in the present study is evaluated as a substrate for lignocellulolytic enzyme production and for the production of ethanol by the mesophilic fungus Fusarium oxysporum under submerged conditions, implementing a consolidated bioconversion process. Fermentation experiments were performed with sugar mixtures simulating the carbohydrate content of BG in order to determine the utilization pattern that could be expected during the fermentation of the cellulose and hemicellulose hydrolysate of BG. The sugar mixture fermentation study focused on the effect of the initial total sugar concentration and on the effect of the aeration rate on fermenting performance of F. oxysporum. The alkali pretreatment of BG and different aeration levels during the ethanol production stage were studied for the optimization of the ethanol production by F. oxysporum. Results: Enzyme yields as high as 550, 22.5, 6.5, 3225, 0.3, 1.25 and 3U per g of carbon source of endoglucanase, cellobiohydrolase, β-D-glucosidase, xylanase, feruloyl esterase, β-D-xylosidase and α-L-arabinofuranosidase respectively, were obtained during the growth stage under optimized submerged conditions. An ethanol yield of 109 g ethanol per kg of dry BG was obtained with alkali-pretreated BG under microaerobic conditions (0.01 vvm), corresponding to 60% of the theoretical yield based on total glucose and xylose content of BG. Conclusion: The enzymatic profile of the extracellular extract from F. oxysporum submerged cultures using BG and corn cob as the carbon source was proved efficient for a successful hydrolysis of BG. The fermentation study carried out using sugar mixtures simulating BG's carbohydrates content and consecutively alkali-pretreated and untreated BG, indicates that BG hydrolysis is the bottleneck of the bioconversion process. However, a considerable bioconversion yield was achieved (60% of the theoretical) making this bioprocess worthy of further investigation for a potential commercial application. © 2009 Xiros and Christakopoulos; licensee BioMed Central Ltd.	en
heal.publisher	BIOMED CENTRAL LTD	en
heal.journalName	Biotechnology for Biofuels	en
dc.identifier.doi	10.1186/1754-6834-2-4	en
dc.identifier.isi	ISI:000272006900001	en
dc.identifier.volume	2	en