Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system

Xiros, C; Moukouli, M; Topakas, E; Christakopoulos, P

dc.contributor.author	Xiros, C	en
dc.contributor.author	Moukouli, M	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:30:38Z
dc.date.available	2014-03-01T01:30:38Z
dc.date.issued	2009	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19608
dc.subject	Brewer's spent grain	en
dc.subject	Ferulic acid	en
dc.subject	Feruloyl esterase	en
dc.subject	End-product inhibition	en
dc.subject	Protease	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	P-COUMARIC ACIDS	en
dc.subject.other	HYDROXYCINNAMIC ACIDS	en
dc.subject.other	ASPERGILLUS-NIGER	en
dc.subject.other	PHENOLIC-ACIDS	en
dc.subject.other	ESTERASES	en
dc.subject.other	FERMENTATION	en
dc.subject.other	PURIFICATION	en
dc.subject.other	HYDROLYSIS	en
dc.subject.other	ENZYMES	en
dc.title	Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2009.06.018	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2009.06.018	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	In this study, the factors affecting ferulic acid (FA) release from Brewer's spent grain (BSG), by the crude enzyme extract of Fusarium oxysporum were investigated. In order to evaluate the importance of the multienzyme preparation on FA release, the synergistic action of feruloyl esterase (FAE. FoFaeC-12213) and xylanase (Trichoderma longibrachiatum M3) monoenzymes was studied. More than double amount of FA release (1 mg g(-1) dry BSG) was observed during hydrolytic reactions by the crude enzyme extract compared to hydrolysis by the monoenzymes (0.37 mg g(-1) dry BSG). The protease content of the crude extract and the inhibitory effect of FA as an end-product were also evaluated concerning their effect on FA release. The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release. (C) 2009 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	BIORESOURCE TECHNOLOGY	en
dc.identifier.doi	10.1016/j.biortech.2009.06.018	en
dc.identifier.isi	ISI:000269812600056	en
dc.identifier.volume	100	en
dc.identifier.issue	23	en
dc.identifier.spage	5917	en
dc.identifier.epage	5921	en