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Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system

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dc.contributor.author Xiros, C en
dc.contributor.author Moukouli, M en
dc.contributor.author Topakas, E en
dc.contributor.author Christakopoulos, P en
dc.date.accessioned 2014-03-01T01:30:38Z
dc.date.available 2014-03-01T01:30:38Z
dc.date.issued 2009 en
dc.identifier.issn 0960-8524 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/19608
dc.subject Brewer's spent grain en
dc.subject Ferulic acid en
dc.subject Feruloyl esterase en
dc.subject End-product inhibition en
dc.subject Protease en
dc.subject.classification Agricultural Engineering en
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.classification Energy & Fuels en
dc.subject.other P-COUMARIC ACIDS en
dc.subject.other HYDROXYCINNAMIC ACIDS en
dc.subject.other ASPERGILLUS-NIGER en
dc.subject.other PHENOLIC-ACIDS en
dc.subject.other ESTERASES en
dc.subject.other FERMENTATION en
dc.subject.other PURIFICATION en
dc.subject.other HYDROLYSIS en
dc.subject.other ENZYMES en
dc.title Factors affecting ferulic acid release from Brewer's spent grain by Fusarium oxysporum enzymatic system en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.biortech.2009.06.018 en
heal.identifier.secondary http://dx.doi.org/10.1016/j.biortech.2009.06.018 en
heal.language English en
heal.publicationDate 2009 en
heal.abstract In this study, the factors affecting ferulic acid (FA) release from Brewer's spent grain (BSG), by the crude enzyme extract of Fusarium oxysporum were investigated. In order to evaluate the importance of the multienzyme preparation on FA release, the synergistic action of feruloyl esterase (FAE. FoFaeC-12213) and xylanase (Trichoderma longibrachiatum M3) monoenzymes was studied. More than double amount of FA release (1 mg g(-1) dry BSG) was observed during hydrolytic reactions by the crude enzyme extract compared to hydrolysis by the monoenzymes (0.37 mg g(-1) dry BSG). The protease content of the crude extract and the inhibitory effect of FA as an end-product were also evaluated concerning their effect on FA release. The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release. (C) 2009 Elsevier Ltd. All rights reserved. en
heal.publisher ELSEVIER SCI LTD en
heal.journalName BIORESOURCE TECHNOLOGY en
dc.identifier.doi 10.1016/j.biortech.2009.06.018 en
dc.identifier.isi ISI:000269812600056 en
dc.identifier.volume 100 en
dc.identifier.issue 23 en
dc.identifier.spage 5917 en
dc.identifier.epage 5921 en


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