Feruloyl esterase-catalysed synthesis of glycerol sinapate using ionic liquids mixtures

Vafiadi, C; Topakas, E; Nahmias, VR; Faulds, CB; Christakopoulos, P

dc.contributor.author	Vafiadi, C	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Nahmias, VR	en
dc.contributor.author	Faulds, CB	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:30:41Z
dc.date.available	2014-03-01T01:30:41Z
dc.date.issued	2009	en
dc.identifier.issn	0168-1656	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19613
dc.subject	Feruloyl esterase	en
dc.subject	Ionic liquids	en
dc.subject	Glycerol sinapate	en
dc.subject	LDL oxidative modification	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	LOW-DENSITY-LIPOPROTEIN	en
dc.subject.other	ANTARCTICA LIPASE-B	en
dc.subject.other	ASPERGILLUS-NIGER	en
dc.subject.other	ANTIOXIDANT ACTIVITY	en
dc.subject.other	PHENOLIC-ACIDS	en
dc.subject.other	ENZYMATIC-SYNTHESIS	en
dc.subject.other	HUMICOLA-INSOLENS	en
dc.subject.other	ESTERIFICATION	en
dc.subject.other	TRANSESTERIFICATION	en
dc.subject.other	PURIFICATION	en
dc.title	Feruloyl esterase-catalysed synthesis of glycerol sinapate using ionic liquids mixtures	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jbiotec.2008.08.008	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.jbiotec.2008.08.008	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	The ability of a feruloyl esterase (AnFaeA). either in free or immobilised (cross-linked enzyme aggregates) form, to catalyse the esterification of glycerol, a major by-product of the biodiesel industry, with sinapic acid was studied in four hexafluorophosphate anion-containing ionic liquids: ((Bmiml[PF6], [Omim][PF6], [C(2)OHmim][PF6] and [C(5)O(2)mim][PF6]). Such ionic liquids are considered 'green' reaction systems. The synthetic reaction was optimised in [C(2)OHmim][PF6] and the highest conversion yield was 72.5 +/- 2.1%, while, at the same reaction conditions in [C(5)O(2)mim] [PF6], a similar conversion yield was obtained (76.7 +/- 1.5%). AnFaeA was active in its free and immobilised form, with the latter retaining a part of its synthetic activity after 5 consecutive 24 h-period reaction cycles. Sinapic acid was esterified to one of the primary hydroxyl groups of glycerol and retained, after esterification, 63.1 +/- 0.3% and 89.5 +/- 1.1% of its antioxidant activity against low-density lipoprotein oxidation, when added at concentrations of 10 and 60 mu M, respectively, in the assay mixture. (c) 2008 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	JOURNAL OF BIOTECHNOLOGY	en
dc.identifier.doi	10.1016/j.jbiotec.2008.08.008	en
dc.identifier.isi	ISI:000262795600020	en
dc.identifier.volume	139	en
dc.identifier.issue	1	en
dc.identifier.spage	124	en
dc.identifier.epage	129	en