Fractionation of proteins and carbohydrates of extracellular polymeric substances in a membrane bioreactor system

Malamis, S; Andreadakis, A

dc.contributor.author	Malamis, S	en
dc.contributor.author	Andreadakis, A	en
dc.date.accessioned	2014-03-01T01:30:45Z
dc.date.available	2014-03-01T01:30:45Z
dc.date.issued	2009	en
dc.identifier.issn	0960-8524	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19623
dc.subject	EPS	en
dc.subject	Fouling	en
dc.subject	Fractionation	en
dc.subject	Membrane bioreactor	en
dc.subject	SMP	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Biomass activities	en
dc.subject.other	EPS	en
dc.subject.other	Extracellular polymeric substances	en
dc.subject.other	Membrane bioreactor	en
dc.subject.other	Membrane bioreactor systems	en
dc.subject.other	Molecular weight cutoffs	en
dc.subject.other	Operating conditions	en
dc.subject.other	Operational problems	en
dc.subject.other	Sludge retentions	en
dc.subject.other	Small molecules	en
dc.subject.other	SMP	en
dc.subject.other	Bioconversion	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Industrial chemicals	en
dc.subject.other	Macromolecules	en
dc.subject.other	Membranes	en
dc.subject.other	Proteins	en
dc.subject.other	Shape memory effect	en
dc.subject.other	Supramolecular chemistry	en
dc.subject.other	Membrane fouling	en
dc.subject.other	carbohydrate	en
dc.subject.other	polymer	en
dc.subject.other	protein	en
dc.subject.other	bioactivity	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	carbohydrate	en
dc.subject.other	fouling	en
dc.subject.other	fractionation	en
dc.subject.other	membrane	en
dc.subject.other	polymer	en
dc.subject.other	protein	en
dc.subject.other	sludge	en
dc.subject.other	article	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	decomposition	en
dc.subject.other	fouling control	en
dc.subject.other	macromolecule	en
dc.subject.other	membrane filter	en
dc.subject.other	molecular weight	en
dc.subject.other	priority journal	en
dc.subject.other	protein analysis	en
dc.subject.other	sludge	en
dc.subject.other	solubility	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Biotechnology	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Equipment Design	en
dc.subject.other	Macromolecular Substances	en
dc.subject.other	Membranes, Artificial	en
dc.subject.other	Nitrogen	en
dc.subject.other	Permeability	en
dc.subject.other	Polymers	en
dc.subject.other	Proteins	en
dc.subject.other	Quaternary Ammonium Compounds	en
dc.subject.other	Reproducibility of Results	en
dc.subject.other	Time Factors	en
dc.subject.other	Water Purification	en
dc.title	Fractionation of proteins and carbohydrates of extracellular polymeric substances in a membrane bioreactor system	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2009.01.053	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biortech.2009.01.053	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	The major operational problem associated with membrane bioreactors (MBR) is membrane fouling, for which extracellular polymeric substances (EPS) are primarily responsible. In this work both the soluble and bound EPS (i.e. SMP and EPS) produced in an MBR system operating under sludge retention times (SRT) of 10, 15, 20 and 33 days were fractionized by means of membranes having variable molecular weight cutoffs (300 kDa, 100 kDa, 10 kDa & 1 kDa). The results show that increasing the SRT leads to a reduction of SMP and EPS and that these reductions are more pronounced for the SRTs in the range 10-20 days. This reduction is more significant for carbohydrates than for proteins. The decrease of SMP and EPS with increasing SRT from 10 to 20 days led to a significant decrease of the level of fouling. The further increase of SRT to 33 days did not significantly impact on the level of fouling as the SMP and EPS concentrations did not change much. Under the examined operating conditions, EPS were found to be composed mainly of large macromolecules having a size of 0.45 mu m-300 kDa and to a lower extent of very small molecules (< 1 kDa) that are not easily decomposed by the biomass activity. The majority of SMP is composed of very small molecules (< 1 kDa), while some macromolecules in the range of 0.45 mu m-300 kDa are present. Consequently, both EPS and SMP were found to have a bimodal character. (C) 2009 Elsevier Ltd. All rights reserved.	en
heal.publisher	ELSEVIER SCI LTD	en
heal.journalName	Bioresource Technology	en
dc.identifier.doi	10.1016/j.biortech.2009.01.053	en
dc.identifier.isi	ISI:000265810900026	en
dc.identifier.volume	100	en
dc.identifier.issue	13	en
dc.identifier.spage	3350	en
dc.identifier.epage	3357	en