Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems

Tzialla, AA; Taha, AA; Kalogeris, E; Stamatis, H

dc.contributor.author	Tzialla, AA	en
dc.contributor.author	Taha, AA	en
dc.contributor.author	Kalogeris, E	en
dc.contributor.author	Stamatis, H	en
dc.date.accessioned	2014-03-01T01:30:53Z
dc.date.available	2014-03-01T01:30:53Z
dc.date.issued	2009	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19668
dc.subject	Laccases	en
dc.subject	Stabilization	en
dc.subject	Ternary systems	en
dc.subject	Terpenes	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Botrytis cinerea	en
dc.subject.other	Catalytic efficiencies	en
dc.subject.other	Catalytic performance	en
dc.subject.other	D-limonene	en
dc.subject.other	Fungal laccases	en
dc.subject.other	Laccases	en
dc.subject.other	Monoterpenes	en
dc.subject.other	Non-conventional media	en
dc.subject.other	Reaction media	en
dc.subject.other	Reaction system	en
dc.subject.other	Residual enzymes	en
dc.subject.other	Terpenes	en
dc.subject.other	Tert butanol	en
dc.subject.other	Trametes versicolor	en
dc.subject.other	Catalysts	en
dc.subject.other	Enzyme activity	en
dc.subject.other	Enzymes	en
dc.subject.other	Lipids	en
dc.subject.other	Olefins	en
dc.subject.other	Reaction rates	en
dc.subject.other	Stabilization	en
dc.subject.other	Water content	en
dc.subject.other	Ternary systems	en
dc.subject.other	Botryotinia fuckeliana	en
dc.subject.other	Fungi	en
dc.subject.other	Trametes versicolor	en
dc.subject.other	fungal protein	en
dc.subject.other	laccase	en
dc.subject.other	solvent	en
dc.subject.other	terpene	en
dc.subject.other	tert butyl alcohol	en
dc.subject.other	water	en
dc.subject.other	article	en
dc.subject.other	Botrytis	en
dc.subject.other	chemistry	en
dc.subject.other	enzyme stability	en
dc.subject.other	enzymology	en
dc.subject.other	isolation and purification	en
dc.subject.other	metabolism	en
dc.subject.other	temperature	en
dc.subject.other	time	en
dc.subject.other	Trametes	en
dc.subject.other	Botrytis	en
dc.subject.other	Enzyme Stability	en
dc.subject.other	Fungal Proteins	en
dc.subject.other	Laccase	en
dc.subject.other	Monoterpenes	en
dc.subject.other	Solvents	en
dc.subject.other	Temperature	en
dc.subject.other	tert-Butyl Alcohol	en
dc.subject.other	Time Factors	en
dc.subject.other	Trametes	en
dc.subject.other	Water	en
dc.title	Improving the catalytic performance of fungal laccases in monoterpene-based reaction systems	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10529-009-0014-5	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s10529-009-0014-5	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Ternary systems consisting of monoterpenes (α-pinene or D-limonene), tert-butanol and water were used as reaction media to enhance the catalytic performance of laccases from various fungi sources (Trametes versicolor, T. hirsuta and Botrytis cinerea). The enzymes had improved catalytic efficiency (5- to 10-fold) in α-pinene-rich environment, while optimal reaction rates were in high-water content systems (15.5% v/v). The stability of laccases was significantly improved in monoterpene-based systems (up to 90% residual enzyme activity after 24 h at 30°C) in comparison with other non-conventional media. The results indicate that these ternary systems can increase the potential of laccases as catalysts for various oxidations. © Springer Science+Business Media B.V. 2009.	en
heal.publisher	SPRINGER	en
heal.journalName	Biotechnology Letters	en
dc.identifier.doi	10.1007/s10529-009-0014-5	en
dc.identifier.isi	ISI:000268726500021	en
dc.identifier.volume	31	en
dc.identifier.issue	9	en
dc.identifier.spage	1451	en
dc.identifier.epage	1456	en