Line optical tweezers: A tool to induce transformations in stained liposomes and to estimate shear modulus

Spyratou, E; Mourelatou, EA; Georgopoulos, A; Demetzos, C; Makropoulou, M; Serafetinides, AA

dc.contributor.author	Spyratou, E	en
dc.contributor.author	Mourelatou, EA	en
dc.contributor.author	Georgopoulos, A	en
dc.contributor.author	Demetzos, C	en
dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Serafetinides, AA	en
dc.date.accessioned	2014-03-01T01:30:59Z
dc.date.available	2014-03-01T01:30:59Z
dc.date.issued	2009	en
dc.identifier.issn	0927-7757	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19709
dc.subject	Fission	en
dc.subject	Line optical tweezers	en
dc.subject	Shear modulus	en
dc.subject	Stained liposomes	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.other	Bioactive molecules	en
dc.subject.other	Cellular process	en
dc.subject.other	Drug delivery system	en
dc.subject.other	Drug encapsulation	en
dc.subject.other	Fission	en
dc.subject.other	Line optical tweezers	en
dc.subject.other	Non-contact	en
dc.subject.other	Optical force	en
dc.subject.other	Physicochemical property	en
dc.subject.other	Shape deformation	en
dc.subject.other	Shear modulus	en
dc.subject.other	Stained liposomes	en
dc.subject.other	State transitions	en
dc.subject.other	Cell membranes	en
dc.subject.other	Cytology	en
dc.subject.other	Deformation	en
dc.subject.other	Drug delivery	en
dc.subject.other	Drug interactions	en
dc.subject.other	Elastic moduli	en
dc.subject.other	Encapsulation	en
dc.subject.other	Liposomes	en
dc.subject.other	Optical instruments	en
dc.subject.other	Phospholipids	en
dc.subject.other	Shear strain	en
dc.subject.other	Optical tweezers	en
dc.subject.other	liposome	en
dc.subject.other	phosphatidylcholine	en
dc.subject.other	article	en
dc.subject.other	cell activity	en
dc.subject.other	cell membrane	en
dc.subject.other	cell vacuole	en
dc.subject.other	chemical interaction	en
dc.subject.other	controlled study	en
dc.subject.other	drug delivery system	en
dc.subject.other	encapsulation	en
dc.subject.other	laser	en
dc.subject.other	optical tweezers	en
dc.subject.other	phase transition	en
dc.subject.other	physical chemistry	en
dc.subject.other	priority journal	en
dc.subject.other	shear stress	en
dc.title	Line optical tweezers: A tool to induce transformations in stained liposomes and to estimate shear modulus	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.colsurfa.2009.07.049	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.colsurfa.2009.07.049	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Liposomes have been actively studied as models of cell membranes and are currently used as drug delivery systems of bioactive molecules. Liposome transformations that mimic cellular processes and are associated with their physicochemical properties have become field of interest the last decade. However, there has been little experimental work on controlled vesicle transformations by non-contact, optical handling methods. In this paper we present the use of line optical tweezers to observe liposome state transitions and transformations. Dynamic shape deformations were induced by line optical tweezers in giant stained liposomes leading to budding transition, fission and pearling creation. Under the controlled effect of line optical tweezers reversible liposome deformations were observed. The shear modulus mu of the membrane was inferred by measuring deformation of stained liposomes induced by the applied optical force. Further laser radiation caused irreversible shape deformations of liposomes, which were transformed from spherical to tubular vesicles. The ability of the selective manipulation of liposomes brings us closer to study their physicochemical properties which play a key role in cellular-liposome interactions, drug encapsulation and delivery efficiency. (C) 2009 Elsevier B. V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Colloids and Surfaces A: Physicochemical and Engineering Aspects	en
dc.identifier.doi	10.1016/j.colsurfa.2009.07.049	en
dc.identifier.isi	ISI:000272541600005	en
dc.identifier.volume	349	en
dc.identifier.issue	1-3	en
dc.identifier.spage	35	en
dc.identifier.epage	42	en