Microimaging FT-IR spectroscopy on pathological breast tissues

Anastassopoulou, J; Boukaki, E; Conti, C; Ferraris, P; Giorgini, E; Rubini, C; Sabbatini, S; Theophanides, T; Tosi, G

dc.contributor.author	Anastassopoulou, J	en
dc.contributor.author	Boukaki, E	en
dc.contributor.author	Conti, C	en
dc.contributor.author	Ferraris, P	en
dc.contributor.author	Giorgini, E	en
dc.contributor.author	Rubini, C	en
dc.contributor.author	Sabbatini, S	en
dc.contributor.author	Theophanides, T	en
dc.contributor.author	Tosi, G	en
dc.date.accessioned	2014-03-01T01:31:05Z
dc.date.available	2014-03-01T01:31:05Z
dc.date.issued	2009	en
dc.identifier.issn	0924-2031	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19740
dc.subject	Breast cancer	en
dc.subject	Cluster analysis	en
dc.subject	Microimaging FT-IR spectroscopy	en
dc.subject	Pattern recognition analysis	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.classification	Chemistry, Physical	en
dc.subject.classification	Spectroscopy	en
dc.subject.other	Abnormal spectra	en
dc.subject.other	Amide I	en
dc.subject.other	Breast cancer	en
dc.subject.other	Breast tissues	en
dc.subject.other	Cancer progression	en
dc.subject.other	Chemometric method	en
dc.subject.other	Conformational change	en
dc.subject.other	FTIR spectroscopy	en
dc.subject.other	Helix conformation	en
dc.subject.other	Hierarchical clustering analysis	en
dc.subject.other	Histological analysis	en
dc.subject.other	Hypomethylation	en
dc.subject.other	Malignant tissues	en
dc.subject.other	Membrane fluidity	en
dc.subject.other	Micro-imaging	en
dc.subject.other	Microimaging FT-IR spectroscopy	en
dc.subject.other	Normal tissue	en
dc.subject.other	Normal zone	en
dc.subject.other	Secondary structures	en
dc.subject.other	Solid cancer	en
dc.subject.other	Vibrational modes	en
dc.subject.other	Amides	en
dc.subject.other	Cluster analysis	en
dc.subject.other	Convolution	en
dc.subject.other	Fourier transform infrared spectroscopy	en
dc.subject.other	Histology	en
dc.subject.other	Nucleic acids	en
dc.subject.other	Pattern recognition	en
dc.subject.other	Principal component analysis	en
dc.subject.other	Proteins	en
dc.subject.other	Spectroscopic analysis	en
dc.subject.other	Spectroscopy	en
dc.subject.other	Spectrum analysis	en
dc.title	Microimaging FT-IR spectroscopy on pathological breast tissues	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.vibspec.2009.07.005	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.vibspec.2009.07.005	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Microimaging Fourier transform infrared spectroscopy is able to monitor differentiation between normal and malignant tissues. All the specimens, previously submitted to histological analysis, displayed abnormal spectra compared with the corresponding normal tissues with changes in many diagnostic bands like those arising from phosphate, C-O and CH stretching vibrational modes. The comparison between cancer (K) and connective (C) spectra evidenced the following differences: in the nu CH region 3000-2800 cm(-1) no hypomethylation effect was evident in K: the convolution of the bands of connective indicated an expected higher membrane fluidity; in the neoplastic zone, Amide I and II modes showed convoluted bands with maxima at 1651 and 1547 cm(-1), respectively, indicating an alpha-helix conformation of proteins due to changes in the secondary structure proteins upon carcinogenesis. Other signature bands, such as the deformation O-P-O phosphate band at 965 cm(-1), suggested DNA conformational changes in solid cancer, infiltrating cancer and neoplasia in the region 1350-800 cm(-1). These characteristic bands have been monitored as a function of the degree of cancer progression. Chemometric methods, such as principal component analysis (PCA) and hierarchical clustering analysis (HCA) have been used in order to distinguish spectra of neoplastic and normal zones. (C) 2009 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Vibrational Spectroscopy	en
dc.identifier.doi	10.1016/j.vibspec.2009.07.005	en
dc.identifier.isi	ISI:000272124100019	en
dc.identifier.volume	51	en
dc.identifier.issue	2	en
dc.identifier.spage	270	en
dc.identifier.epage	275	en