Protective effects of mastic oil from pistacia lentiscus variation chia against experimental growth of lewis lung carcinoma

Magkouta, S; Stathopoulos, GT; Psallidas, I; Papapetropoulos, A; Kolisis, FN; Roussos, C; Loutrari, H

dc.contributor.author	Magkouta, S	en
dc.contributor.author	Stathopoulos, GT	en
dc.contributor.author	Psallidas, I	en
dc.contributor.author	Papapetropoulos, A	en
dc.contributor.author	Kolisis, FN	en
dc.contributor.author	Roussos, C	en
dc.contributor.author	Loutrari, H	en
dc.date.accessioned	2014-03-01T01:31:43Z
dc.date.available	2014-03-01T01:31:43Z
dc.date.issued	2009	en
dc.identifier.issn	0163-5581	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/19906
dc.subject	Lewis Lung Carcinoma	en
dc.subject.classification	Oncology	en
dc.subject.classification	Nutrition & Dietetics	en
dc.subject.other	chemokine	en
dc.subject.other	essential oil	en
dc.subject.other	guanosine triphosphatase	en
dc.subject.other	immunoglobulin enhancer binding protein	en
dc.subject.other	mastic oil	en
dc.subject.other	perillyl alcohol	en
dc.subject.other	pinene	en
dc.subject.other	Ras protein	en
dc.subject.other	RhoA guanine nucleotide binding protein	en
dc.subject.other	unclassified drug	en
dc.subject.other	vasculotropin	en
dc.subject.other	vegetable oil	en
dc.subject.other	animal cell	en
dc.subject.other	animal experiment	en
dc.subject.other	animal model	en
dc.subject.other	animal tissue	en
dc.subject.other	antineoplastic activity	en
dc.subject.other	apoptosis	en
dc.subject.other	article	en
dc.subject.other	cancer cell	en
dc.subject.other	cancer inhibition	en
dc.subject.other	cancer prevention	en
dc.subject.other	controlled study	en
dc.subject.other	dose response	en
dc.subject.other	drug efficacy	en
dc.subject.other	drug mechanism	en
dc.subject.other	enzyme linked immunosorbent assay	en
dc.subject.other	gene expression	en
dc.subject.other	immunocompetence	en
dc.subject.other	immunohistochemistry	en
dc.subject.other	in vitro study	en
dc.subject.other	in vivo study	en
dc.subject.other	Lewis carcinoma	en
dc.subject.other	male	en
dc.subject.other	mouse	en
dc.subject.other	nonhuman	en
dc.subject.other	Pistacia lentiscus	en
dc.subject.other	protein expression	en
dc.subject.other	reporter gene	en
dc.subject.other	signal transduction	en
dc.subject.other	tumor vascularization	en
dc.subject.other	tumor volume	en
dc.subject.other	Angiogenesis Inducing Agents	en
dc.subject.other	Animals	en
dc.subject.other	Antineoplastic Agents	en
dc.subject.other	Apoptosis	en
dc.subject.other	Carcinoma, Lewis Lung	en
dc.subject.other	Cell Line, Tumor	en
dc.subject.other	Cell Proliferation	en
dc.subject.other	Cell Survival	en
dc.subject.other	Inflammation Mediators	en
dc.subject.other	Male	en
dc.subject.other	Medicine, Traditional	en
dc.subject.other	Mice	en
dc.subject.other	Mice, Inbred C57BL	en
dc.subject.other	Monoterpenes	en
dc.subject.other	Neoplasm Transplantation	en
dc.subject.other	Phytotherapy	en
dc.subject.other	Pistacia	en
dc.subject.other	Plant Oils	en
dc.subject.other	Signal Transduction	en
dc.subject.other	Tumor Burden	en
dc.subject.other	Mus	en
dc.subject.other	Pistacia lentiscus	en
dc.title	Protective effects of mastic oil from pistacia lentiscus variation chia against experimental growth of lewis lung carcinoma	en
heal.type	journalArticle	en
heal.identifier.primary	10.1080/01635580902825647	en
heal.identifier.secondary	http://dx.doi.org/10.1080/01635580902825647	en
heal.language	English	en
heal.publicationDate	2009	en
heal.abstract	Mastic oil from Pistacia lentiscus variation chia, a traditionally used dietary flavoring agent with medicinal properties, has been shown to exert in vitro antitumor activities, but no study has addressed in vivo efficacy and mechanisms of action. Presently, we demonstrated that treatment of immunocompetent mice with mastic oil (45 mg/kg body weight, intraperitoneally, 3 times a wk for similar to 3 wk) significantly inhibited tumor growth (56.4% +/- 5.7 maximum reduction in tumor volumes) without toxicity. Analysis of tumors by immunohistochemistry and ELISA indicated that this effect is associated with increased apoptosis, reduced neovascularization, and inhibition of chemokine expression. Likewise mastic oil reduced vascular endothelial growth factor and chemokine release by Lewis lung carcinoma (LLC) cells. Furthermore, mastic oil administration decreased small guanosine triphosphatases (GTPases) Ras, RhoA and nuclear factor-kappa-B-dependent reporter gene expression in vivo and in vitro, indicating a mechanistic link between mastic oil activities and blocking of relevant signaling and transcription pathways. A dose-response comparison with perillyl alcohol and alpha-pinene, two of its components, revealed a higher efficacy of mastic oil, pointing to a beneficial collective interaction among its ingredients. Conclusively, our results provide novel in vivo evidence of mastic oil inhibitory effects on tumor growth and set a rational basis for its future application in cancer prevention.	en
heal.publisher	LAWRENCE ERLBAUM ASSOC INC-TAYLOR & FRANCIS	en
heal.journalName	Nutrition and Cancer	en
dc.identifier.doi	10.1080/01635580902825647	en
dc.identifier.isi	ISI:000270427100009	en
dc.identifier.volume	61	en
dc.identifier.issue	5	en
dc.identifier.spage	640	en
dc.identifier.epage	648	en