Biomimetic chromatographic analysis of selenium species: Application for the estimation of their pharmacokinetic properties

Tsopelas, F; Tsantili-Kakoulidou, A; Ochsenkuhn-Petropoulou, M

dc.contributor.author	Tsopelas, F	en
dc.contributor.author	Tsantili-Kakoulidou, A	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.date.accessioned	2014-03-01T01:32:57Z
dc.date.available	2014-03-01T01:32:57Z
dc.date.issued	2010	en
dc.identifier.issn	1618-2642	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20246
dc.subject	Biomimetic chromatography	en
dc.subject	Human serum albumin/α1-acid glycoprotein columns	en
dc.subject	Immobilized artificial membrane chromatography	en
dc.subject	Oral absorption	en
dc.subject	Plasma protein binding	en
dc.subject	Selenium speciation	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Buffer composition	en
dc.subject.other	DL-methionine	en
dc.subject.other	Electrostatic interactions	en
dc.subject.other	Human serum albumins	en
dc.subject.other	Immobilized artificial membrane chromatographies	en
dc.subject.other	Immobilized artificial membranes	en
dc.subject.other	Mobile phase conditions	en
dc.subject.other	Octanol-water partitioning	en
dc.subject.other	Oral absorption	en
dc.subject.other	Pharmacokinetic properties	en
dc.subject.other	Phosphate-buffered salines	en
dc.subject.other	Plasma protein	en
dc.subject.other	Plasma protein binding	en
dc.subject.other	Retention behavior	en
dc.subject.other	Retention factors	en
dc.subject.other	Selenides	en
dc.subject.other	Selenium speciation	en
dc.subject.other	Selenocystamine	en
dc.subject.other	Selenocystine	en
dc.subject.other	Selenourea	en
dc.subject.other	Stationary phasis	en
dc.subject.other	Absorption	en
dc.subject.other	Acids	en
dc.subject.other	Biochemistry	en
dc.subject.other	Body fluids	en
dc.subject.other	Chromatographic analysis	en
dc.subject.other	Drug products	en
dc.subject.other	Glycoproteins	en
dc.subject.other	Imaging techniques	en
dc.subject.other	Liquid chromatography	en
dc.subject.other	Plasmas	en
dc.subject.other	Proteins	en
dc.subject.other	Radiation protection	en
dc.subject.other	Selenium	en
dc.subject.other	Selenium compounds	en
dc.subject.other	Biomimetics	en
dc.subject.other	organoselenium derivative	en
dc.subject.other	orosomucoid	en
dc.subject.other	selenic acid	en
dc.subject.other	selenium	en
dc.subject.other	selenium derivative	en
dc.subject.other	serum albumin	en
dc.subject.other	sodium selenite	en
dc.subject.other	absorption	en
dc.subject.other	article	en
dc.subject.other	artificial membrane	en
dc.subject.other	biological model	en
dc.subject.other	biomimetics	en
dc.subject.other	chemistry	en
dc.subject.other	human	en
dc.subject.other	liquid chromatography	en
dc.subject.other	methodology	en
dc.subject.other	Absorption	en
dc.subject.other	Biomimetics	en
dc.subject.other	Chromatography, Liquid	en
dc.subject.other	Humans	en
dc.subject.other	Membranes, Artificial	en
dc.subject.other	Models, Biological	en
dc.subject.other	Organoselenium Compounds	en
dc.subject.other	Orosomucoid	en
dc.subject.other	Selenium	en
dc.subject.other	Selenium Compounds	en
dc.subject.other	Serum Albumin	en
dc.subject.other	Sodium Selenite	en
dc.title	Biomimetic chromatographic analysis of selenium species: Application for the estimation of their pharmacokinetic properties	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00216-010-3624-9	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00216-010-3624-9	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	The retention behavior of selenites, selenates, seleno-dl-methionine, selenocystine, selenocystamine, selenourea, dimethyl selenide, and dimethyl diselenide was investigated by means of biomimetic liquid chromatography. For this purpose, two immobilized artificial membrane (IAM) columns, namely, IAM.PC.DD2 and IAM.PC.MG, and two immobilized plasma protein columns, human serum albumin (HSA) and α1-acid glycoprotein (AGP) columns, were employed using different mobile phase conditions in respect to pH and buffer composition. In general, satisfactory interrelations between retention factors obtained with the two IAM stationary phases and HSA/AGP columns were obtained. Large differences were observed between biomimetic retention factors and octanol-water logD values, since the latter fail to describe electrostatic interactions. In contrast, despite the column diversity, the net retention outcome on all four biomimetic columns was quite similar, especially in the presence of phosphate-buffered saline, which by its effective shielding alleviates the differences between the stationary phases. Of the two IAM columns, IAM.PC.DD2 showed better performance when compared with HSA and AGP columns as well as to octanol-water partitioning. Biomimetic chromatographic indices were further used to estimate the percentage of human oral absorption and plasma protein binding of the eight selenium species investigated, according to equations previously reported in the literature. The estimated values of human oral absorption imply moderate absorption only for dimethyl diselenide, which also may exhibit considerable plasma protein binding. Moderate affinity for plasma proteins should also be expected for dimethyl selenide and selenocystamine. © 2010 Springer-Verlag.	en
heal.publisher	SPRINGER HEIDELBERG	en
heal.journalName	Analytical and Bioanalytical Chemistry	en
dc.identifier.doi	10.1007/s00216-010-3624-9	en
dc.identifier.isi	ISI:000279453000018	en
dc.identifier.volume	397	en
dc.identifier.issue	6	en
dc.identifier.spage	2171	en
dc.identifier.epage	2180	en