Carbohydrate esterases of family 2 are 6-O-deacetylases

Topakas, E; Kyriakopoulos, S; Biely, P; Hirsch, J; Vafiadi, C; Christakopoulos, P

dc.contributor.author	Topakas, E	en
dc.contributor.author	Kyriakopoulos, S	en
dc.contributor.author	Biely, P	en
dc.contributor.author	Hirsch, J	en
dc.contributor.author	Vafiadi, C	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:32:58Z
dc.date.available	2014-03-01T01:32:58Z
dc.date.issued	2010	en
dc.identifier.issn	0014-5793	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20260
dc.subject	Acetyl esterase	en
dc.subject	Transesterification	en
dc.subject	CE-2 family	en
dc.subject	6-O-Deacetylase	en
dc.subject	Cellvibrio japonicus	en
dc.subject	Clostridium thermocellum	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biophysics	en
dc.subject.classification	Cell Biology	en
dc.subject.other	REESEI ACETYL ESTERASE	en
dc.subject.other	ALTERNATIVE SYNTHESES	en
dc.subject.other	METHYLATED SUGARS	en
dc.subject.other	KONJAC GLUCOMANNAN	en
dc.subject.other	COUPLED ASSAY	en
dc.title	Carbohydrate esterases of family 2 are 6-O-deacetylases	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.febslet.2009.11.095	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.febslet.2009.11.095	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	Three acetyl esterases (AcEs) from the saprophytic bacteria Cellvibrio japonicus and Clostridium thermocellum, members of the carbohydrate esterase (CE) family 2, were tested for their activity against a series of model substrates including partially acetylated gluco-, manno- and xylopyranosides. All three enzymes showed a strong preference for deacetylation of the 6-position in aldohexoses. This regioselectivity is different from that of typical acetylxylan esterases (AcXEs). In aqueous medium saturated with vinyl acetate, the CE-2 enzymes catalyzed transacetylation to the same position, i.e., to the primary hydroxyl group of mono-and disaccharides. Xylose and xylooligosaccharides did not serve as acetyl group acceptors, therefore the CE-2 enzymes appear to be 6-O-deacetylases. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	FEBS LETTERS	en
dc.identifier.doi	10.1016/j.febslet.2009.11.095	en
dc.identifier.isi	ISI:000273858600014	en
dc.identifier.volume	584	en
dc.identifier.issue	3	en
dc.identifier.spage	543	en
dc.identifier.epage	548	en