Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)

Economopoulou, C; Pappa, V; Papageorgiou, S; Kontsioti, F; Economopoulou, P; Charitidou, E; Girkas, K; Kapsimali, V; Papasteriadi, C; Tsirigotis, P; Papageorgiou, E; Dervenoulas, J; Economopoulos, T

dc.contributor.author	Economopoulou, C	en
dc.contributor.author	Pappa, V	en
dc.contributor.author	Papageorgiou, S	en
dc.contributor.author	Kontsioti, F	en
dc.contributor.author	Economopoulou, P	en
dc.contributor.author	Charitidou, E	en
dc.contributor.author	Girkas, K	en
dc.contributor.author	Kapsimali, V	en
dc.contributor.author	Papasteriadi, C	en
dc.contributor.author	Tsirigotis, P	en
dc.contributor.author	Papageorgiou, E	en
dc.contributor.author	Dervenoulas, J	en
dc.contributor.author	Economopoulos, T	en
dc.date.accessioned	2014-03-01T01:32:59Z
dc.date.available	2014-03-01T01:32:59Z
dc.date.issued	2010	en
dc.identifier.issn	0939-5555	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20263
dc.subject	Apoptosis	en
dc.subject	Cell cycle	en
dc.subject	CMML	en
dc.subject	Cyclin D1	en
dc.subject	Myelodysplastic syndromes	en
dc.subject.classification	Hematology	en
dc.subject.other	caspase 2	en
dc.subject.other	caspase 3	en
dc.subject.other	caspase 5	en
dc.subject.other	caspase 6	en
dc.subject.other	caspase 7	en
dc.subject.other	caspase 8	en
dc.subject.other	cyclin B	en
dc.subject.other	cyclin C	en
dc.subject.other	cyclin D1	en
dc.subject.other	cyclin D2	en
dc.subject.other	cyclin dependent kinase 1	en
dc.subject.other	cyclin dependent kinase 2	en
dc.subject.other	cyclin dependent kinase 4	en
dc.subject.other	cycline	en
dc.subject.other	granzyme B	en
dc.subject.other	lipocortin 5	en
dc.subject.other	messenger RNA	en
dc.subject.other	protein bcl xl	en
dc.subject.other	protein mcl 1	en
dc.subject.other	protein p21	en
dc.subject.other	ribonuclease	en
dc.subject.other	unclassified drug	en
dc.subject.other	apoptosis regulatory protein	en
dc.subject.other	cell cycle protein	en
dc.subject.other	acute granulocytic leukemia	en
dc.subject.other	adult	en
dc.subject.other	aged	en
dc.subject.other	apoptosis	en
dc.subject.other	article	en
dc.subject.other	cell cycle	en
dc.subject.other	cell cycle S phase	en
dc.subject.other	chronic myelomonocytic leukemia	en
dc.subject.other	controlled study	en
dc.subject.other	cytogenetics	en
dc.subject.other	female	en
dc.subject.other	flow cytometry	en
dc.subject.other	gene expression	en
dc.subject.other	human	en
dc.subject.other	major clinical study	en
dc.subject.other	male	en
dc.subject.other	myelodysplastic syndrome	en
dc.subject.other	pathogenesis	en
dc.subject.other	priority journal	en
dc.subject.other	prognosis	en
dc.subject.other	refractory anemia	en
dc.subject.other	refractory anemia with excess blasts	en
dc.subject.other	refractory anemia with excess blasts in transformation	en
dc.subject.other	refractory anemia with ringed sideroblasts	en
dc.subject.other	RNA extraction	en
dc.subject.other	gene expression regulation	en
dc.subject.other	genetics	en
dc.subject.other	karyotyping	en
dc.subject.other	middle aged	en
dc.subject.other	pathology	en
dc.subject.other	Aged	en
dc.subject.other	Aged, 80 and over	en
dc.subject.other	Apoptosis	en
dc.subject.other	Apoptosis Regulatory Proteins	en
dc.subject.other	Cell Cycle Proteins	en
dc.subject.other	Female	en
dc.subject.other	Gene Expression Regulation	en
dc.subject.other	Humans	en
dc.subject.other	Karyotyping	en
dc.subject.other	Male	en
dc.subject.other	Middle Aged	en
dc.subject.other	Myelodysplastic Syndromes	en
dc.subject.other	S Phase	en
dc.title	Cell cycle and apoptosis regulatory gene expression in the bone marrow of patients with de novo myelodysplastic syndromes (MDS)	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00277-009-0835-2	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s00277-009-0835-2	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	Deregulation of cell cycle and apoptosis pathways are known contributors to the pathogenesis ofmyelodysplastic syndromes (MDS). However, the underlying mechanisms are not fully clarified. The aim of our study was to examine mRNA expression levels of cell cycle and apoptosis regulatory genes, as well as the percentage of apoptotic and S phase cells and to correlate the findings with clinical characteristics and prognosis. Sixty patients with MDS, classified according to FAB (17 RA, five RARS, 19 RAEB, nine RAEBT, ten CMML) and WHO (ten RA, three RARS, seven RCMD, two RCMD-RS, 11 RAEBI, eight RAEBII, ten CMML, and nine AML) were included in the study. We found increased expression of anti-apoptotic bclxL and mcl1 genes and decreased expression of p21 gene inMDS patients. Moreover, we found increased expression of anti-apoptotic mcl1 gene in patients with higher than Intermediate-1 IPSS group. Multivariate analysis confirmed that combined expression of apoptotic caspases 8, 3, 6, 5, 2, 7, and Granzyme B was decreased in MDS patients. Regarding cell cycle regulatory genes expression, we demonstrated increased expression of cyclin D1 in patients with CMML Increased combined expression of cyclins B, C, D1, and D2 was found in patients with cytogenetic abnormalities. The two pathways seem to be interconnected as shown by the positive correlation between CDKs 1, 2, 4, p21 and the level of apoptosis and positive correlation between apoptotic caspase 3 expression and the percentage of S phase cells. In conclusion, our study showed altered expression of genes involved in apoptosis and cell cycle in MDS and increased expression of cyclin D1 in patients with CMML. © Springer-Verlag 2009.	en
heal.publisher	SPRINGER	en
heal.journalName	Annals of Hematology	en
dc.identifier.doi	10.1007/s00277-009-0835-2	en
dc.identifier.isi	ISI:000274685400003	en
dc.identifier.volume	89	en
dc.identifier.issue	4	en
dc.identifier.spage	349	en
dc.identifier.epage	358	en