HEAL DSpace

Detection of DNA mutations using a capacitive micro-membrane array

Αποθετήριο DSpace/Manakin

Εμφάνιση απλής εγγραφής

dc.contributor.author Tsouti, V en
dc.contributor.author Boutopoulos, C en
dc.contributor.author Andreakou, P en
dc.contributor.author Ioannou, M en
dc.contributor.author Zergioti, I en
dc.contributor.author Goustouridis, D en
dc.contributor.author Kafetzopoulos, D en
dc.contributor.author Tsoukalas, D en
dc.contributor.author Normand, P en
dc.contributor.author Chatzandroulis, S en
dc.date.accessioned 2014-03-01T01:33:08Z
dc.date.available 2014-03-01T01:33:08Z
dc.date.issued 2010 en
dc.identifier.issn 0956-5663 en
dc.identifier.uri https://dspace.lib.ntua.gr/xmlui/handle/123456789/20337
dc.subject Capacitive biosensor en
dc.subject DNA sensing en
dc.subject Micro-membrane en
dc.subject.classification Biophysics en
dc.subject.classification Biotechnology & Applied Microbiology en
dc.subject.classification Chemistry, Analytical en
dc.subject.classification Electrochemistry en
dc.subject.classification Nanoscience & Nanotechnology en
dc.subject.other Biological interactions en
dc.subject.other Biosensor arrays en
dc.subject.other Capacitive biosensors en
dc.subject.other Capacitive readout en
dc.subject.other Complementary strand en
dc.subject.other DNA hybridization en
dc.subject.other DNA molecules en
dc.subject.other DNA mutation en
dc.subject.other DNA sensing en
dc.subject.other DNA strands en
dc.subject.other Flexible membranes en
dc.subject.other Membrane surface en
dc.subject.other Micro-membrane en
dc.subject.other Single nucleotides en
dc.subject.other Surface stress en
dc.subject.other Ultra-thin en
dc.subject.other Ultra-thin membranes en
dc.subject.other DNA en
dc.subject.other Membranes en
dc.subject.other Oligonucleotides en
dc.subject.other Sensor arrays en
dc.subject.other Surface properties en
dc.subject.other Biosensors en
dc.subject.other DNA en
dc.subject.other nucleic acid en
dc.subject.other oligonucleotide en
dc.subject.other silicon en
dc.subject.other article en
dc.subject.other base mispairing en
dc.subject.other beta thalassemia en
dc.subject.other biosensor en
dc.subject.other controlled study en
dc.subject.other DNA determination en
dc.subject.other DNA hybridization en
dc.subject.other DNA probe en
dc.subject.other DNA strand en
dc.subject.other mutational analysis en
dc.subject.other nucleic acid immobilization en
dc.subject.other nucleotide sequence en
dc.subject.other surface stress en
dc.subject.other beta-Thalassemia en
dc.subject.other Biosensing Techniques en
dc.subject.other DNA en
dc.subject.other DNA Mutational Analysis en
dc.subject.other Equipment Design en
dc.subject.other Humans en
dc.subject.other Microtechnology en
dc.subject.other Mutation en
dc.subject.other Nucleic Acid Hybridization en
dc.subject.other Oligonucleotide Array Sequence Analysis en
dc.subject.other Oligonucleotides en
dc.subject.other Silicon en
dc.subject.other Surface Properties en
dc.title Detection of DNA mutations using a capacitive micro-membrane array en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.bios.2010.07.119 en
heal.identifier.secondary http://dx.doi.org/10.1016/j.bios.2010.07.119 en
heal.language English en
heal.publicationDate 2010 en
heal.abstract The detection of DNA hybridization using capacitive readout and a biosensor array of ultrathin Si membranes is presented. The biosensor exploits the ability of the ultrathin membranes to deflect upon surface stress variations caused by biological interactions. Probe DNA molecules are immobilized on the membrane surface and the surface stress variations during hybridization with their complementary strands force the membrane to deflect and effectively change the capacitance between the flexible membrane and the fixed substrate. The sensor array comprises 256 such sensing sites thus allowing the concurrent sensing of multiple DNA mutations. The biosensor and its performance for the detection of complementary DNA strands are demonstrated using beta-thalassemia oligonucleotides. The experimental results show that the presented sensors are able to detect DNA hybridization and to discriminate single nucleotide mismatches. (C) 2010 Elsevier B.V. All rights reserved. en
heal.publisher ELSEVIER ADVANCED TECHNOLOGY en
heal.journalName Biosensors and Bioelectronics en
dc.identifier.doi 10.1016/j.bios.2010.07.119 en
dc.identifier.isi ISI:000286403400068 en
dc.identifier.volume 26 en
dc.identifier.issue 4 en
dc.identifier.spage 1588 en
dc.identifier.epage 1592 en


Αρχεία σε αυτό το τεκμήριο

Αρχεία Μέγεθος Μορφότυπο Προβολή

Δεν υπάρχουν αρχεία που σχετίζονται με αυτό το τεκμήριο.

Αυτό το τεκμήριο εμφανίζεται στην ακόλουθη συλλογή(ές)

Εμφάνιση απλής εγγραφής