Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture

Papaspyridi, LM; Katapodis, P; Gonou-Zagou, Z; Kapsanaki-Gotsi, E; Christakopoulos, P

dc.contributor.author	Papaspyridi, LM	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Gonou-Zagou, Z	en
dc.contributor.author	Kapsanaki-Gotsi, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:34:02Z
dc.date.available	2014-03-01T01:34:02Z
dc.date.issued	2010	en
dc.identifier.issn	1369-703X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20659
dc.subject	Pleurotus ostreatus	en
dc.subject	FF Biolog MicroPlate	en
dc.subject	Response surface methodology	en
dc.subject	Bioreactor	en
dc.subject	Dietary fibres	en
dc.subject	Glucan	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Engineering, Chemical	en
dc.subject.other	CORN STEEP LIQUOR	en
dc.subject.other	REGIUM FR. SINGER	en
dc.subject.other	OYSTER MUSHROOM	en
dc.subject.other	NUTRITIONAL-VALUE	en
dc.subject.other	MYCELIAL BIOMASS	en
dc.subject.other	NITROGEN-SOURCES	en
dc.subject.other	MANNITOL CYCLE	en
dc.subject.other	FOOD-PRODUCTS	en
dc.subject.other	CARBON SOURCE	en
dc.subject.other	AMINO-ACID	en
dc.title	Optimization of biomass production with enhanced glucan and dietary fibres content by Pleurotus ostreatus ATHUM 4438 under submerged culture	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.bej.2010.04.008	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.bej.2010.04.008	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	This work was aimed at optimizing biomass production by the edible basidiomycete Pleurotus ostreatus ATHUM 4438 in a submerged process with enhanced glucan and dietary fibres content. beta-Glucan from Pleurotus sp. (pleuran) has been used as food supplements due to its immunosuppressive activity. Like other dietary fibre components, oyster mushroom polysaccharides can stimulate the growth of colon microorganisms (probiotics), i.e. act as prebiotics. We used the FF MicroPlate for substrate utilization and growth monitoring. The pattern of substrate catabolism forms a substrate assimilation fingerprint which is useful in selecting media components for media optimization of maximum biomass production. Different carbon sources (95) were used and then 8 of them were tested in shake flask cultures. The effect of various organic and complex nitrogen sources on biomass production was also examined and response surface methodology based on central composite design was applied to explore the optimal medium composition. When the optimized culture medium was tested in a 20-L stirred tank bioreactor, using 57g L-1 xylose and 37g L-1 corn steep liquor, high yields (39.2g L-1) of dry biomass was obtained. The yield coefficients for total Oilcan and dietary fibres on mycelial biomass formed were 140 +/- 4 and 625 +/- 9 mg g(-1) mycelium dry weight, respectively. (C) 2010 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE SA	en
heal.journalName	BIOCHEMICAL ENGINEERING JOURNAL	en
dc.identifier.doi	10.1016/j.bej.2010.04.008	en
dc.identifier.isi	ISI:000279061400008	en
dc.identifier.volume	50	en
dc.identifier.issue	3	en
dc.identifier.spage	131	en
dc.identifier.epage	138	en