Void volume markers in reversed-phase and biomimetic liquid chromatography

Tsopelas, F; Ochsenkuhn-Petropoulou, M; Tsantili-Kakoulidou, A

dc.contributor.author	Tsopelas, F	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.contributor.author	Tsantili-Kakoulidou, A	en
dc.date.accessioned	2014-03-01T01:34:50Z
dc.date.available	2014-03-01T01:34:50Z
dc.date.issued	2010	en
dc.identifier.issn	0021-9673	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/20890
dc.subject	Biomimetic chromatography	en
dc.subject	C18/IAM/HSA/AGP columns	en
dc.subject	Retention factor	en
dc.subject	Reversed-phase liquid chromatography	en
dc.subject	Void volume markers	en
dc.subject.classification	Biochemical Research Methods	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Buffer composition	en
dc.subject.other	C/IAM/HSA/AGP columns	en
dc.subject.other	Chromatographic column	en
dc.subject.other	Chromatographic conditions	en
dc.subject.other	Eluents	en
dc.subject.other	High purity water	en
dc.subject.other	Human serum albumins	en
dc.subject.other	Immobilized artificial membranes	en
dc.subject.other	Immobilized proteins	en
dc.subject.other	Mobile phase	en
dc.subject.other	Mobile phasis	en
dc.subject.other	Retention factors	en
dc.subject.other	Reversed phase	en
dc.subject.other	Reversed-phase liquid chromatography	en
dc.subject.other	Stationary phase	en
dc.subject.other	Void volume	en
dc.subject.other	Acids	en
dc.subject.other	Biomimetics	en
dc.subject.other	Liquids	en
dc.subject.other	Liquid chromatography	en
dc.subject.other	acetic acid	en
dc.subject.other	ascorbic acid	en
dc.subject.other	citric acid	en
dc.subject.other	cysteine	en
dc.subject.other	formic acid	en
dc.subject.other	human serum albumin	en
dc.subject.other	orosomucoid	en
dc.subject.other	oxalic acid	en
dc.subject.other	glycoprotein	en
dc.subject.other	serum albumin	en
dc.subject.other	accuracy	en
dc.subject.other	analytic method	en
dc.subject.other	article	en
dc.subject.other	biomimetics	en
dc.subject.other	chemical analysis	en
dc.subject.other	chemical composition	en
dc.subject.other	chemical structure	en
dc.subject.other	controlled study	en
dc.subject.other	pH	en
dc.subject.other	priority journal	en
dc.subject.other	protein analysis	en
dc.subject.other	protein determination	en
dc.subject.other	purification	en
dc.subject.other	reversed phase liquid chromatography	en
dc.subject.other	separation technique	en
dc.subject.other	chemistry	en
dc.subject.other	human	en
dc.subject.other	instrumentation	en
dc.subject.other	liquid chromatography	en
dc.subject.other	standard	en
dc.subject.other	Chromatography, Liquid	en
dc.subject.other	Glycoproteins	en
dc.subject.other	Humans	en
dc.subject.other	Reference Standards	en
dc.subject.other	Serum Albumin	en
dc.title	Void volume markers in reversed-phase and biomimetic liquid chromatography	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.chroma.2010.02.062	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.chroma.2010.02.062	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	A comparative investigation of 15 void volume marker candidates was carried out on two reversed-phase (RP), two immobilized artificial membrane (IAM) and two immobilized protein columns, namely Human Serum Albumin (HSA) and alpha(1)-acid-glycoprotein (AGP), using different mobile phases in respect to pH and buffer composition. Pycnometric analysis of the 6 chromatographic columns was also employed for reasons of comparison. The results revealed the possible overestimation of stationary phase void volume if a general void volume marker is used under different chromatographic conditions. In particular, overestimation of the investigated columns' void volumes was a common phenomenon when using acidic eluents or High Purity Water (HPW) as mobile phase. For this purpose, a classification of the recommended chemicals according to the column type or the pH of the mobile phase is suggested for the accurate determination of retention factors. (C) 2010 Elsevier B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	Journal of Chromatography A	en
dc.identifier.doi	10.1016/j.chroma.2010.02.062	en
dc.identifier.isi	ISI:000276975200013	en
dc.identifier.volume	1217	en
dc.identifier.issue	17	en
dc.identifier.spage	2847	en
dc.identifier.epage	2854	en