Biomass, laccase and endoglucanase production by Lentinula edodes during solid state fermentation of reed grass, bean stalks and wheat straw residues

Philippoussis, A; Diamantopoulou, P; Papadopoulou, K; Lakhtar, H; Roussos, S; Parissopoulos, G; Papanikolaou, S

dc.contributor.author	Philippoussis, A	en
dc.contributor.author	Diamantopoulou, P	en
dc.contributor.author	Papadopoulou, K	en
dc.contributor.author	Lakhtar, H	en
dc.contributor.author	Roussos, S	en
dc.contributor.author	Parissopoulos, G	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-03-01T01:35:22Z
dc.date.available	2014-03-01T01:35:22Z
dc.date.issued	2011	en
dc.identifier.issn	0959-3993	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21018
dc.subject	Chemical constituents	en
dc.subject	Correlations	en
dc.subject	Endoglucanase	en
dc.subject	Enzyme activities	en
dc.subject	Glucosamine	en
dc.subject	Growth rate	en
dc.subject	Laccase	en
dc.subject	Lentinula edodes	en
dc.subject	Lignocellulosic residues	en
dc.subject	Mycelial biomass	en
dc.subject	Solid state fermentation	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Chemical constituents	en
dc.subject.other	Correlations	en
dc.subject.other	Endoglucanase	en
dc.subject.other	Glucosamines	en
dc.subject.other	Laccases	en
dc.subject.other	Lentinula edodes	en
dc.subject.other	Lignocellulosic residues	en
dc.subject.other	Mycelial biomass	en
dc.subject.other	Solid state fermentation	en
dc.subject.other	Biomass	en
dc.subject.other	Ecology	en
dc.subject.other	Enzymes	en
dc.subject.other	Fermentation	en
dc.subject.other	Growth rate	en
dc.subject.other	Strain	en
dc.subject.other	Substrates	en
dc.subject.other	Lentinula edodes	en
dc.subject.other	Triticum aestivum	en
dc.title	Biomass, laccase and endoglucanase production by Lentinula edodes during solid state fermentation of reed grass, bean stalks and wheat straw residues	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11274-010-0458-8	en
heal.identifier.secondary	http://dx.doi.org/10.1007/s11274-010-0458-8	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	Mycelium growth rates, biomass concentration (estimated as glucosamine content) and laccase and endoglucanase secretion were monitored during solid state fermentation (SSF) of wheat straw (WS), reed grass (RG) and bean stalk (BS) residues by Lentinula edodes strains 119, 121, and 122. In a first experiment, these strains were subjected to screening regarding their growth rates and biomass yield, where strain 121 proved to be the fastest colonizer. However, the greater biomass yield at the end of colonization was demonstrated by strain 122 on BS (465.93 mg g-1 d.w.). In a second experiment, growth characters, as well as endoglucanase and laccase production patterns of the selected strains 121 and 122 were monitored at three intervals i.e., at 33, 66, and 100% of substrate colonization. BS furnished the highest endoglucanase production for strain 121, while RG for strain 122. A strain and substrate-dependent behaviour of the enzyme secretion was detected, with strain 122 presenting maximal endoglucanase activity in all substrates at the initial (33%) and final (100%) stages of colonization (0.64-0.90 and 0.79-0.97 U g-1, respectively). However, in strain 121 the peak of endoglucanase production was detected in the early stages of colonization (at 33% on WS and at 66% on RG and BS). Laccase activity showed increased values (maxima on WS, 353. 68 and 548. 67 U g-1 by strains 121 and 122, respectively) at 66% of colonization. Correlation analysis of growth data demonstrated negative relations between growth rate and biomass yield and between laccase and endoglucanase activities on WS and RG substrates fermented by strain 122. Finally, possible relations of growth parameters with nutritional constituents of the substrates were investigated. © 2010 Springer Science+Business Media B.V.	en
heal.publisher	SPRINGER	en
heal.journalName	World Journal of Microbiology and Biotechnology	en
dc.identifier.doi	10.1007/s11274-010-0458-8	en
dc.identifier.isi	ISI:000286113200011	en
dc.identifier.volume	27	en
dc.identifier.issue	2	en
dc.identifier.spage	285	en
dc.identifier.epage	297	en