Development of an electrochemical biosensor for the rapid detection of cholera toxin using air stable lipid films with incorporated ganglioside GM1

Nikoleli, G-P; Nikolelis, DP; Tzamtzis, N

dc.contributor.author	Nikoleli, G-P	en
dc.contributor.author	Nikolelis, DP	en
dc.contributor.author	Tzamtzis, N	en
dc.date.accessioned	2014-03-01T01:35:30Z
dc.date.available	2014-03-01T01:35:30Z
dc.date.issued	2011	en
dc.identifier.issn	1040-0397	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21087
dc.subject	Biosensors	en
dc.subject	Cholera toxin	en
dc.subject	Electrochemistry	en
dc.subject	Gangliosides	en
dc.subject	Stabilized lipid films	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.classification	Electrochemistry	en
dc.subject.other	SURFACE-PLASMON RESONANCE	en
dc.subject.other	BINDING PROTEIN-1 RECEPTOR	en
dc.subject.other	NAPHTHALENE ACETIC-ACID	en
dc.subject.other	OPTICAL SPOT-TEST	en
dc.subject.other	LIPOSOMES	en
dc.subject.other	MONOLAYERS	en
dc.subject.other	MEMBRANES	en
dc.subject.other	BILAYERS	en
dc.subject.other	FRUITS	en
dc.title	Development of an electrochemical biosensor for the rapid detection of cholera toxin using air stable lipid films with incorporated ganglioside GM1	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elan.201100307	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elan.201100307	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	The present work describes the investigations of electrochemical interactions of cholera toxin with stabilized lipid films supported on a methacrylate polymer on a glass fiber filter with incorporated ganglioside GM1 for the development of a biosensor for cholera toxin. The analyte was injected into the flowing streams of a carrier electrolyte solution, the flow of the solution stops for 5min and an ion current transient was obtained, the magnitude of which is correlated to the toxin concentration with detection limits of 0.06μM. The method was applied in blood serum samples. Further work is directed to the rapid detection of other toxins used in bioterrorism using this novel ultrathin film technology. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.publisher	WILEY-BLACKWELL	en
heal.journalName	Electroanalysis	en
dc.identifier.doi	10.1002/elan.201100307	en
dc.identifier.isi	ISI:000295143900026	en
dc.identifier.volume	23	en
dc.identifier.issue	9	en
dc.identifier.spage	2182	en
dc.identifier.epage	2187	en