Exploring the elution mechanism of selenium species on liquid chromatography

Tsopelas, F; Tsantili-Kakoulidou, A; Ochsenkuhn-Petropoulou, M

dc.contributor.author	Tsopelas, F	en
dc.contributor.author	Tsantili-Kakoulidou, A	en
dc.contributor.author	Ochsenkuhn-Petropoulou, M	en
dc.date.accessioned	2014-03-01T01:35:44Z
dc.date.available	2014-03-01T01:35:44Z
dc.date.issued	2011	en
dc.identifier.issn	1615-9306	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21164
dc.subject	Biomimetic chromatography	en
dc.subject	IAM chromatography	en
dc.subject	Retention factors	en
dc.subject	RPLC	en
dc.subject	Selenium speciation	en
dc.subject.classification	Chemistry, Analytical	en
dc.subject.other	Chromatographic conditions	en
dc.subject.other	Dimethylselenide	en
dc.subject.other	DL-methionine	en
dc.subject.other	Electrostatic interactions	en
dc.subject.other	Elution mechanism	en
dc.subject.other	IAM chromatography	en
dc.subject.other	Immobilized artificial membranes	en
dc.subject.other	Mobile phase	en
dc.subject.other	Mobile phase conditions	en
dc.subject.other	Net charges	en
dc.subject.other	Partitioning mechanism	en
dc.subject.other	Plasma protein	en
dc.subject.other	Retention factors	en
dc.subject.other	Reversed phase	en
dc.subject.other	RPLC	en
dc.subject.other	Secondary interference	en
dc.subject.other	Selenium speciation	en
dc.subject.other	Selenocystamine	en
dc.subject.other	Selenocystine	en
dc.subject.other	Selenourea	en
dc.subject.other	Stationary phasis	en
dc.subject.other	Water distribution coefficients	en
dc.subject.other	Amino acids	en
dc.subject.other	Biomimetics	en
dc.subject.other	pH effects	en
dc.subject.other	Principal component analysis	en
dc.subject.other	Selenium	en
dc.subject.other	Selenium compounds	en
dc.subject.other	Water supply systems	en
dc.subject.other	Liquid chromatography	en
dc.subject.other	dimethyl selenide	en
dc.subject.other	octanol	en
dc.subject.other	plasma protein	en
dc.subject.other	selenate	en
dc.subject.other	selenite	en
dc.subject.other	selenium	en
dc.subject.other	selenium derivative	en
dc.subject.other	selenocystine	en
dc.subject.other	selenomethionine	en
dc.subject.other	analytic method	en
dc.subject.other	article	en
dc.subject.other	artificial membrane	en
dc.subject.other	elution	en
dc.subject.other	molecular interaction	en
dc.subject.other	pH	en
dc.subject.other	principal component analysis	en
dc.subject.other	priority journal	en
dc.subject.other	reversed phase liquid chromatography	en
dc.subject.other	Chromatography, Liquid	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Molecular Structure	en
dc.subject.other	Organoselenium Compounds	en
dc.title	Exploring the elution mechanism of selenium species on liquid chromatography	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/jssc.201000771	en
heal.identifier.secondary	http://dx.doi.org/10.1002/jssc.201000771	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	In the present work, the chromatographic behavior of eight selenium species, namely selenites (Se(IV)), selenates (Se(VI)), seleno-DL-methionine (Se-Met), selenocystine (Se-Cyst), selenocystamine (Se-CM), selenourea (Se-U), dimethylselenide ((CH3)2Se) and dimethyldiselenide ((CH3)2Se2), was investigated under different stationary and mobile phase conditions, in an effort to unravel secondary interferences in their underlying elution mechanism. For this purpose, two end-capped and a polar-embedded reversed-phase stationary phases were employed using different mobile phase conditions. Retention factors (log kw) were compared with octanol-water distribution coefficients (log D) as well as with log kw values on two immobilized artificial membrane (IAM) columns and two immobilized artificial plasma proteins stationary phases, obtained in our previous work. The role of electrostatic interactions was confirmed by introducing the net charge of the investigated Se species as an additional term in the log kw/log D interrelation, which in most cases proved to be statistically significant. Principal component analysis of retention factors on all stationary phases and octanol-water log D values, however, showed that the elution of the investigated selenium species is mainly governed by partitioning mechanism under all different chromatographic conditions, while the pH of the mobile phase and the special column characteristics have only a minor effect. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.publisher	WILEY-BLACKWELL	en
heal.journalName	Journal of Separation Science	en
dc.identifier.doi	10.1002/jssc.201000771	en
dc.identifier.isi	ISI:000287942000003	en
dc.identifier.volume	34	en
dc.identifier.issue	4	en
dc.identifier.spage	376	en
dc.identifier.epage	384	en