Growth and biomass production with enhanced β-glucan and dietary fibre contents of Ganoderma australe ATHUM 4345 in a batch-stirred tank bioreactor

Papaspyridi, L-M; Katapodis, P; Gonou-Zagou, Z; Kapsanaki-Gotsi, E; Christakopoulos, P

dc.contributor.author	Papaspyridi, L-M	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Gonou-Zagou, Z	en
dc.contributor.author	Kapsanaki-Gotsi, E	en
dc.contributor.author	Christakopoulos, P	en
dc.date.accessioned	2014-03-01T01:35:46Z
dc.date.available	2014-03-01T01:35:46Z
dc.date.issued	2011	en
dc.identifier.issn	1618-0240	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21191
dc.subject	Bioreactor	en
dc.subject	Dietary fibers	en
dc.subject	Ganoderma australe	en
dc.subject	Glucan	en
dc.subject	Response surface methodology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	Biomass productions	en
dc.subject.other	Biomass yield	en
dc.subject.other	Carbon and nitrogen	en
dc.subject.other	Carbon source	en
dc.subject.other	Central composite designs	en
dc.subject.other	Culture medium	en
dc.subject.other	Dietary fibers	en
dc.subject.other	Dietary fibre	en
dc.subject.other	Dry weight	en
dc.subject.other	Ganoderma	en
dc.subject.other	Ganoderma australe	en
dc.subject.other	Glucan	en
dc.subject.other	Glucans	en
dc.subject.other	Growth monitoring	en
dc.subject.other	Media optimization	en
dc.subject.other	Microplates	en
dc.subject.other	Nutraceuticals	en
dc.subject.other	Optimum concentration	en
dc.subject.other	Response surface methodology	en
dc.subject.other	Shake-flask cultures	en
dc.subject.other	Stirred tank bioreactors	en
dc.subject.other	Yeast extracts	en
dc.subject.other	Yield coefficient	en
dc.subject.other	Bioconversion	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Fibers	en
dc.subject.other	Fungi	en
dc.subject.other	Glucose	en
dc.subject.other	Substrates	en
dc.subject.other	Surface properties	en
dc.subject.other	Tanks (containers)	en
dc.subject.other	Ecology	en
dc.subject.other	beta glucan	en
dc.subject.other	glucose	en
dc.subject.other	nitrogen	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	catabolism	en
dc.subject.other	concentration (composition)	en
dc.subject.other	fungus	en
dc.subject.other	growth rate	en
dc.subject.other	pharmaceutical industry	en
dc.subject.other	polysaccharide	en
dc.subject.other	article	en
dc.subject.other	biomass production	en
dc.subject.other	carbon source	en
dc.subject.other	chemical composition	en
dc.subject.other	controlled study	en
dc.subject.other	dietary fiber	en
dc.subject.other	Ganoderma	en
dc.subject.other	Ganoderma australe	en
dc.subject.other	nonhuman	en
dc.subject.other	plant growth	en
dc.subject.other	productivity	en
dc.subject.other	response surface method	en
dc.subject.other	stirred reactor	en
dc.subject.other	yeast	en
dc.subject.other	Ganoderma australe	en
dc.title	Growth and biomass production with enhanced β-glucan and dietary fibre contents of Ganoderma australe ATHUM 4345 in a batch-stirred tank bioreactor	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.201000102	en
heal.identifier.secondary	http://dx.doi.org/10.1002/elsc.201000102	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	In this study we maximized biomass production by the basidiomycete Ganoderma australe ATHUM 4345, a species of pharmaceutical interest as it is a valuable source of nutraceuticals, including dietary fibers and glucans. We used the Biolog FF MicroPlate to screen 95 different carbon sources for growth monitoring. The pattern of substrate catabolism forms a substrate assimilation fingerprint, which is useful in selecting components for media optimization of maximum biomass production. Response surface methodology, based on the central composite design was applied to explore the optimum concentrations of carbon and nitrogen sources of culture medium in shake flask cultures. When the improved culture medium was tested in a 20-L stirred tank bioreactor, using 13.7g/L glucose and 30.0g/L yeast extract, high biomass yields (10.1±0.4g/L) and productivity of 0.09gL-1h-1 were obtained. The yield coefficients for total glucan and dietary fibers on biomass formed were 94.82±6 and 341.15±12.3mg/g mycelium dry weight, respectively. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.publisher	WILEY-V C H VERLAG GMBH	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.doi	10.1002/elsc.201000102	en
dc.identifier.isi	ISI:000287671800009	en
dc.identifier.volume	11	en
dc.identifier.issue	1	en
dc.identifier.spage	65	en
dc.identifier.epage	74	en