Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw

Panagiotou, G; Topakas, E; Moukouli, M; Christakopoulos, P; Olsson, L

dc.contributor.author	Panagiotou, G	en
dc.contributor.author	Topakas, E	en
dc.contributor.author	Moukouli, M	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Olsson, L	en
dc.date.accessioned	2014-03-01T01:37:10Z
dc.date.available	2014-03-01T01:37:10Z
dc.date.issued	2011	en
dc.identifier.issn	0961-9534	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21468
dc.subject	Consolidated bioprocessing	en
dc.subject	Ethanol	en
dc.subject	Fusarium oxysporum	en
dc.subject	Saccharomyces cerevisiae	en
dc.subject	Wheat straw	en
dc.subject.classification	Agricultural Engineering	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.classification	Energy & Fuels	en
dc.subject.other	Acetyl esterase	en
dc.subject.other	Anaerobic conditions	en
dc.subject.other	Cell mass	en
dc.subject.other	Consolidated bioprocessing	en
dc.subject.other	Corn cob	en
dc.subject.other	Dry matters	en
dc.subject.other	Endoglucanases	en
dc.subject.other	Enzyme mixtures	en
dc.subject.other	Enzyme production	en
dc.subject.other	Ethanol concentrations	en
dc.subject.other	Ethanol production	en
dc.subject.other	Ethanol productivity	en
dc.subject.other	Ethanolic fermentations	en
dc.subject.other	Feruloyl esterase	en
dc.subject.other	Fusarium oxysporums	en
dc.subject.other	Glucosidase	en
dc.subject.other	Mixed cultures	en
dc.subject.other	Novozymes	en
dc.subject.other	Productivity rate	en
dc.subject.other	Saccharomyces cerevisiae F12	en
dc.subject.other	Wheat straws	en
dc.subject.other	Xylanases	en
dc.subject.other	Bioethanol	en
dc.subject.other	Carbohydrates	en
dc.subject.other	Cell culture	en
dc.subject.other	Detoxification	en
dc.subject.other	Enzymes	en
dc.subject.other	Esters	en
dc.subject.other	Ethanol	en
dc.subject.other	Fermentation	en
dc.subject.other	Productivity	en
dc.subject.other	Yeast	en
dc.subject.other	bacterium	en
dc.subject.other	carbohydrate	en
dc.subject.other	enzyme activity	en
dc.subject.other	ethanol	en
dc.subject.other	fermentation	en
dc.subject.other	fungus	en
dc.subject.other	maize	en
dc.subject.other	oxic conditions	en
dc.subject.other	power generation	en
dc.subject.other	recombination	en
dc.subject.other	straw	en
dc.subject.other	substrate	en
dc.subject.other	wheat	en
dc.subject.other	Fusarium oxysporum	en
dc.subject.other	Saccharomyces cerevisiae	en
dc.subject.other	Triticum aestivum	en
dc.subject.other	Zea mays	en
dc.title	Studying the ability of Fusarium oxysporum and recombinant Saccharomyces cerevisiae to efficiently cooperate in decomposition and ethanolic fermentation of wheat straw	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biombioe.2011.05.005	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.biombioe.2011.05.005	en
heal.language	English	en
heal.publicationDate	2011	en
heal.abstract	Fusarium oxysporum F3 alone or in mixed culture with Saccharomyces cerevisiae F12 were used to ferment carbohydrates of wet exploded pre-treated wheat straw (PWS) directly to ethanol. Both microorganisms were first grown aerobically to produce cell mass and thereafter fermented PWS to ethanol under anaerobic conditions. During fermentation, soluble and insoluble carbohydrates were hydrolysed by the lignocellulolytic system of F. oxysporum. Mixed substrate fermentation using PWS and corn cobs (CC) in the ratio 1:2 was used to obtain an enzyme mixture with high cellulolytic and hemicellulolytic activities. Under these conditions, activities as high as 34300, 9100, 326, 24, 169, 27 and 254 U dm(-3) of xylanase, endoglucanase, beta-glucosidase, arabinofuranosidase, avicelase, feruloyl esterase and acetyl esterase, respectively, were obtained. The replacement of the enzyme production phase of F. oxysporum by the addition of commercially available enzymes Celluclast (R) 1.5 L FG and Novozym (R) 188 in 3:1 ratio for the treatment of PWS, resulted in a 3-fold increase in the volumetric ethanol productivity without increasing the ethanol production significantly. By direct bioconversion of 110 kg m(-3) dry matter of PWS, ethanol concentration (4.9 kg m(-3)) and yield (40 g kg(-1) of PWS) were similarly obtained by F. oxysporum and the mixed culture, while productivity rates as high as 34 g m(-3) h(-1) and 108 g m(-3) h(-1) were obtained by F. oxysporum and the mixed culture, respectively. (C) 2011 Elsevier Ltd. All rights reserved.	en
heal.publisher	PERGAMON-ELSEVIER SCIENCE LTD	en
heal.journalName	Biomass and Bioenergy	en
dc.identifier.doi	10.1016/j.biombioe.2011.05.005	en
dc.identifier.isi	ISI:000294938000048	en
dc.identifier.volume	35	en
dc.identifier.issue	8	en
dc.identifier.spage	3727	en
dc.identifier.epage	3732	en