Further synthetic studies with phosphorylated peptides

Theodoropoulos, D; Souchleris, I

dc.contributor.author	Theodoropoulos, D	en
dc.contributor.author	Souchleris, I	en
dc.date.accessioned	2014-03-01T01:37:39Z
dc.date.available	2014-03-01T01:37:39Z
dc.date.issued	1964	en
dc.identifier.issn	00062960	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/21598
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-33947486078&partnerID=40&md5=f5d9dfd54b72b52107b83bcffb810f9c	en
dc.title	Further synthetic studies with phosphorylated peptides	en
heal.type	journalArticle	en
heal.publicationDate	1964	en
heal.abstract	Synthetic carbobenzoxy-β-benzyl-L-aspartyl-L-serylglycine benzyl ester and carbobenzoxy-β-benzyl-L-aspartyl-L-seryl-L-glutamic dibenzyl ester were phosphorylated by means of di-p-nitrobenzylphosphorochloridate in the presence of imidazole in 90% and 50% yield, respectively. The crude phosphate triesters, carbobenzoxy-β-benzyl-L-aspartyl-O-(O,O-di-p-nitrobenzylphospho)-L- serylglycine benzyl ester and carbobenzoxy-β-benzyl-L-aspartyl-O-(O,O-di-p-nitrobenzylphospho)-L-seryl-L- glutamic dibenzyl ester could not be purified by fractional precipitation from usual solvents; however, countercurrent distribution has been found to be a suitable technique for isolating these and related substances in analytically pure form Preliminary experiments on the synthesis of phosphodiesters of serine are also described. In this connection, O-butylphospho-D,L-serine was synthesized and its isolation from by-products, e.g., phosphoserine and serine, was effected by countercurrent distribution.	en
heal.journalName	Biochemistry	en
dc.identifier.volume	3	en
dc.identifier.issue	2	en
dc.identifier.spage	145	en
dc.identifier.epage	151	en