A PURIFIED ALPHA-GALACTOSIDASE FROM ASPERGILLUS-NIGER WITH ENHANCED KINETIC CHARACTERISTICS

AGNANTIARI, G; CHRISTAKOPOULOS, P; KEKOS, D; MACRIS, BJ

dc.contributor.author	AGNANTIARI, G	en
dc.contributor.author	CHRISTAKOPOULOS, P	en
dc.contributor.author	KEKOS, D	en
dc.contributor.author	MACRIS, BJ	en
dc.date.accessioned	2014-03-01T01:40:51Z
dc.date.available	2014-03-01T01:40:51Z
dc.date.issued	1991	en
dc.identifier.issn	0138-4988	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/23278
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	PURIFICATION	en
dc.title	A PURIFIED ALPHA-GALACTOSIDASE FROM ASPERGILLUS-NIGER WITH ENHANCED KINETIC CHARACTERISTICS	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1991	en
heal.abstract	Extracellular alpha-galactosidase from Aspergillus niger was purified 128-fold over the crude extract by gel filtration, ion exchange chromatography and chromatofocusing. Certain substrates and end products affected enzyme activity. Among the former p-nitrophenyl-alpha-galactopyranoside (PNPG) inhibited the enzyme at 1.4 mM while melibiose did not inhibit alpha-galactosidase at concentrations up to 50 mM. Enzymic end products such as glucose did not inhibit the enzyme at concentrations up to 100 mM while galactose exhibited a competitive inhibition with a K(i) = 1.29 mM. The kinetic characteristics of the enzyme compared favourably to other microbial alpha-galactosidases and make it suitable for food process applications.	en
heal.publisher	AKADEMIE VERLAG GMBH	en
heal.journalName	ACTA BIOTECHNOLOGICA	en
dc.identifier.isi	ISI:A1991HF98200008	en
dc.identifier.volume	11	en
dc.identifier.issue	5	en
dc.identifier.spage	479	en
dc.identifier.epage	484	en