MECHANICAL BLOOD TRAUMATIZATION BY TUBING AND THROTTLES IN INVITRO PUMP TESTS - EXPERIMENTAL RESULTS AND IMPLICATIONS FOR HEMOLYSIS THEORY

SCHIMA, H; MULLER, MR; TSANGARIS, S; GHEISEDER, G; SCHLUSCHE, C; LOSERT, U; THOMA, H; WOLNER, E

dc.contributor.author	SCHIMA, H	en
dc.contributor.author	MULLER, MR	en
dc.contributor.author	TSANGARIS, S	en
dc.contributor.author	GHEISEDER, G	en
dc.contributor.author	SCHLUSCHE, C	en
dc.contributor.author	LOSERT, U	en
dc.contributor.author	THOMA, H	en
dc.contributor.author	WOLNER, E	en
dc.date.accessioned	2014-03-01T01:42:12Z
dc.date.available	2014-03-01T01:42:12Z
dc.date.issued	1993	en
dc.identifier.issn	0160-564X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/23714
dc.subject	MECHANICAL HEMOLYSIS	en
dc.subject	SHEAR STRESS	en
dc.subject	BIOCOMPATIBILITY	en
dc.subject	CENTRIFUGAL BLOOD PUMP	en
dc.subject.classification	Engineering, Biomedical	en
dc.subject.classification	Transplantation	en
dc.subject.other	SHEAR-STRESS	en
dc.title	MECHANICAL BLOOD TRAUMATIZATION BY TUBING AND THROTTLES IN INVITRO PUMP TESTS - EXPERIMENTAL RESULTS AND IMPLICATIONS FOR HEMOLYSIS THEORY	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1993	en
heal.abstract	Blood has become essential as a test fluid to evaluate hemolysis and biocompatibility of blood pumps in vitro. The blood is usually pumped from a blood bag into a circuit against elevated pressure. A throttle or a length of tubing is used to produce the pressure head. Blood damage caused by the shear stress in these pressure-reducing devices should be minimal. It is not known whether the high but short-lasting shear stress in a throttle is more or less damaging to the blood than the low but long-lasting stress in tubing. In this study, throttles (width 11 mm, minimal height 0.9 mm, length 30 mm; shear stress = 136 N/m2 lasting for 3.23 ms); and tubing (inner diameter 9.5 mm, length 4.5 m, shear stress = 4.5 N/m2 lasting for 3.5 s) were compared at a flow of 5 L/min and a pressure drop of 150 mm Hg. Experiments (n = 10) with bovine blood were performed in two parallel setups using Bio-Medicus pumps BP80. Free hemoglobin in plasma (fHb) and thromboxane B2 (TXB2) were measured. After 6 h, the fHb increase was 31.9 +/- 19.1 mg% for the throttle setup and 32.3 +/- 16.2 for the tubing setup. The TXB2 release was 296 +/- 70 and 305 +/- 54 pg/0.1 ml respectively after 4 h. In summary, no significant differences between the two setups for either fHb or TXB2 could be detected. So the use of a throttle, which requires far less priming volume and a smaller blood-contacting surface while also offering a wider range of adjustment, seems preferable. Furthermore, in contrast to some other in vitro studies, these results strongly indicate that blood is quite insensitive to high shear stress exposure in the millisecond range even if repetitively applied.	en
heal.publisher	BLACKWELL SCIENCE INC	en
heal.journalName	ARTIFICIAL ORGANS	en
dc.identifier.isi	ISI:A1993KP61100003	en
dc.identifier.volume	17	en
dc.identifier.issue	3	en
dc.identifier.spage	164	en
dc.identifier.epage	170	en