ANTIBODIES TO EYRKK VESICULAR STOMATITIS VIRUS-RELATED PEPTIDE ACCOUNT ONLY FOR A MINORITY OF ANTI-RO60KD ANTIBODIES

ROUTSIAS, JG; SAKARELLOSDAITSIOTIS, M; DETSIKAS, E; TZIOUFAS, AG; SAKARELLOS, C; MOUTSOPOULOS, HM

dc.contributor.author	ROUTSIAS, JG	en
dc.contributor.author	SAKARELLOSDAITSIOTIS, M	en
dc.contributor.author	DETSIKAS, E	en
dc.contributor.author	TZIOUFAS, AG	en
dc.contributor.author	SAKARELLOS, C	en
dc.contributor.author	MOUTSOPOULOS, HM	en
dc.date.accessioned	2014-03-01T01:42:44Z
dc.date.available	2014-03-01T01:42:44Z
dc.date.issued	1994	en
dc.identifier.issn	0009-9104	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/23926
dc.subject	VESICULAR STOMATITIS VIRUS	en
dc.subject	AUTOIMMUNE RHEUMATIC DISEASES	en
dc.subject	SYNTHETIC PEPTIDES	en
dc.subject	RO/SSA	en
dc.subject	SYSTEMIC LUPUS ERYTHEMATOSUS	en
dc.subject.classification	Immunology	en
dc.subject.other	SYSTEMIC LUPUS-ERYTHEMATOSUS	en
dc.subject.other	RO RIBONUCLEOPROTEIN	en
dc.subject.other	SJOGRENS-SYNDROME	en
dc.subject.other	REVISED CRITERIA	en
dc.subject.other	IMMUNOGLOBULIN-G	en
dc.subject.other	PROTEINS	en
dc.subject.other	CLASSIFICATION	en
dc.subject.other	AUTOANTIBODIES	en
dc.subject.other	HETEROGENEITY	en
dc.subject.other	EPITOPES	en
dc.title	ANTIBODIES TO EYRKK VESICULAR STOMATITIS VIRUS-RELATED PEPTIDE ACCOUNT ONLY FOR A MINORITY OF ANTI-RO60KD ANTIBODIES	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	Previous studies demonstrated a possible antigenic relation between the carboxyl terminal portion of anti-Ro60kD autoantigen and a nucleocapsid protein (N) of vesicular stomatitis virus (VSV). In order to investigate whether anti-Ro60kD autoantibodies react with the VSV homologous region of the Ro60kD protein we synthesized, according to Merrifield's method, the EYRKKMDI octapeptide (8p) sharing a common sequence with the N protein of VSV. Sera from 61 patients with autoimmune rheumatic diseases (34 systemic lupus erythematosus (SLE), 21 Sjogren's syndrome (SS) and six rheumatoid arthritis (RA)) as well as 59 from normal blood donors were tested for the presence of anti-Ro60kD autoantibodies by ELISA and immunoblot (IB) and anti-8p antibodies by ELISA. Antibodies to 8p were found in 9/31 of anti-Ro60kD IB-positive sera, 5/30 of anti-Ro60kD-negative sera and 2/59 of normal control sera. The concordance between the anti-8p ELISA and the anti-Ro60kD IB was very poor (chi(2) = 0.71, P = 0.4) in contrast to the anti-Ro60kD ELISA and the anti-Ro IB (chi(2) = 27.6, P = 10(-7)). Subsequent affinity purification of the anti-8p antibodies from a strong positive anti-8p and anti-Ro60kD SLE serum yielded 95% depletion of the anti-8p activity and 37% reduction of the anti-Ro60kD activity. Inhibition assays with the affinity-purified anti-8p antibodies demonstrated that the octapeptide gave 94.5% inhibition of the anti-Ro60kD activity, while Ro60kD protein led to 42.3% inhibition of the anti-8p. Preincubation of the serum with the octapeptide produced 4% inhibition of anti-Ro60kD ELISA. These results indicate that the anti-8p antibodies account only for a minority of the anti-Ro60kD autoantibodies.	en
heal.publisher	BLACKWELL SCIENCE LTD	en
heal.journalName	CLINICAL AND EXPERIMENTAL IMMUNOLOGY	en
dc.identifier.isi	ISI:A1994PW37700011	en
dc.identifier.volume	98	en
dc.identifier.issue	3	en
dc.identifier.spage	414	en
dc.identifier.epage	418	en