ENZYMATIC-SYNTHESIS OF TRISACCHARIDES AND ALKYL BETA-D-GLUCOSIDES BY THE TRANSGLYCOSYLATION REACTION OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM

CHRISTAKOPOULOS, P; BHAT, MK; KEKOS, D; MACRIS, BJ

dc.contributor.author	CHRISTAKOPOULOS, P	en
dc.contributor.author	BHAT, MK	en
dc.contributor.author	KEKOS, D	en
dc.contributor.author	MACRIS, BJ	en
dc.date.accessioned	2014-03-01T01:42:48Z
dc.date.available	2014-03-01T01:42:48Z
dc.date.issued	1994	en
dc.identifier.issn	0141-8130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/23942
dc.subject	TRANSGLYCOSYLATION	en
dc.subject	BETA-GLUCOSIDASE	en
dc.subject	FUSARIUM OXYSPORUM	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	CATALYZED SYNTHESIS	en
dc.subject.other	LACTOSE	en
dc.subject.other	XYLOSIDASE	en
dc.subject.other	HYDROLYSIS	en
dc.title	ENZYMATIC-SYNTHESIS OF TRISACCHARIDES AND ALKYL BETA-D-GLUCOSIDES BY THE TRANSGLYCOSYLATION REACTION OF BETA-GLUCOSIDASE FROM FUSARIUM-OXYSPORUM	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1994	en
heal.abstract	Purified beta-glucosidase from Fusarium oxysporum catalyses hydrolysis and transglycosylation reactions. By utilizing the transglycosylation reaction, trisaccharides and alkyl beta-D-glucosides were synthesized under optimal conditions in the presence of various disaccharides and alcohols. The yields of trisaccharides and alkyl beta-D-glucosides were 22-37% and 10-33% of the total sugar, respectively. The enzyme retained 70-80% of its original activity in the presence of 25% (w/v) methanol, ethanol and propanol. Thus, beta-glucosidase from F. oxysporum appears to be an ideal enzyme for the synthesis of useful trisaccharides and alkyl beta-D-glucosides.	en
heal.publisher	BUTTERWORTH-HEINEMANN LTD	en
heal.journalName	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES	en
dc.identifier.isi	ISI:A1994PZ44800011	en
dc.identifier.volume	16	en
dc.identifier.issue	6	en
dc.identifier.spage	331	en
dc.identifier.epage	334	en