EFFECTS OF ASCORBIC-ACID ON TERT-BUTYL HYDROPEROXIDE-INDUCED DEFORMABILITY DECREASE IN HUMAN ERYTHROCYTES

GALARIS, D; YOVA, D; KORANTZOPOULOS, P; BARBOUNAKI, S; KOUTSOURIS, D

dc.contributor.author	GALARIS, D	en
dc.contributor.author	YOVA, D	en
dc.contributor.author	KORANTZOPOULOS, P	en
dc.contributor.author	BARBOUNAKI, S	en
dc.contributor.author	KOUTSOURIS, D	en
dc.date.accessioned	2014-03-01T01:43:50Z
dc.date.available	2014-03-01T01:43:50Z
dc.date.issued	1995	en
dc.identifier.issn	0271-5198	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24225
dc.subject	RED CELL DEFORMABILITY	en
dc.subject	TERT-BUTYL HYDROPEROXIDE	en
dc.subject	ASCORBIC ACID	en
dc.subject	HUMAN ERYTHROCYTES	en
dc.subject	CELL TRANSIT ANALYZER	en
dc.subject.classification	Hematology	en
dc.subject.other	POTENTIAL PROTECTIVE MECHANISM	en
dc.subject.other	SPERM WHALE MYOGLOBIN	en
dc.subject.other	LIPID-PEROXIDATION	en
dc.subject.other	HEMOGLOBIN	en
dc.subject.other	METMYOGLOBIN	en
dc.subject.other	OXIDATION	en
dc.subject.other	DAMAGE	en
dc.subject.other	INJURY	en
dc.subject.other	MUSCLE	en
dc.title	EFFECTS OF ASCORBIC-ACID ON TERT-BUTYL HYDROPEROXIDE-INDUCED DEFORMABILITY DECREASE IN HUMAN ERYTHROCYTES	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1995	en
heal.abstract	Treatment of human erythrocytes with micromolar concentrations of tert-butyl hydroperoxide causes changes in the physical properties of the cells as evidenced by decreased deformability. The deformability changes followed a lag period which was reciprocally related to the concentrations of hydroperoxide. Ascorbic acid was able to delay these changes or reverse them for a period of time. Oxidation of ascorbic acid was observed, under the same conditions, which was correlated to the ability of both oxy- and met-forms of haemoglobin to catalyse the oxidation of ascorbic acid by tert-butyl hydroperoxide. It seems likely that ascorbic acid acts both as inhibitor of the oxidative activation of haemoglobin, which leads to deformability changes and as substrate for the haemoglobin catalysed tert-butyl hydroperoxide decomposition.	en
heal.publisher	PERGAMON-ELSEVIER SCIENCE LTD	en
heal.journalName	CLINICAL HEMORHEOLOGY	en
dc.identifier.isi	ISI:A1995QD99600012	en
dc.identifier.volume	15	en
dc.identifier.issue	1	en
dc.identifier.spage	107	en
dc.identifier.epage	120	en