Flexibility and thermal denaturation (melting) of irradiated DNA

Kalfas, CA; Loukakis, GK; Georgakilas, AG; Sideris, EG; Anagnostopoulou-Konsta, A

dc.contributor.author	Kalfas, CA	en
dc.contributor.author	Loukakis, GK	en
dc.contributor.author	Georgakilas, AG	en
dc.contributor.author	Sideris, EG	en
dc.contributor.author	Anagnostopoulou-Konsta, A	en
dc.date.accessioned	2014-03-01T01:44:45Z
dc.date.available	2014-03-01T01:44:45Z
dc.date.issued	1996	en
dc.identifier.issn	02183390	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24466
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0004230533&partnerID=40&md5=8773cbce43bf7487e80393d8acb43647	en
dc.subject	Denaturation	en
dc.subject	DNA	en
dc.subject	Flexibility	en
dc.subject	Hyperchromicity	en
dc.subject	Ionising radiation	en
dc.subject	Melting temperature	en
dc.subject	UV absorbance	en
dc.title	Flexibility and thermal denaturation (melting) of irradiated DNA	en
heal.type	journalArticle	en
heal.publicationDate	1996	en
heal.abstract	The study of the melting transition for large molecular weight DNA which had been irradiated with various doses of γ-rays can yield information on the structural and dynamical behaviour of DNA, leading to a better understanding of changes induced by external agents. In order to interpret the accumulated experimental data, a theoretical description capable of correlating thermal changes to those induced by ionizing radiation, is required. The theoretical ideas proposed here, based on first principles and certain assumptions, allow for a quantitative analysis of the melting data and the subsequent evaluation of a very small number of parameters which can be used to interpret the observed variations in experimental quantities (such as UV absorbance) which are related to structural variations.	en
heal.journalName	Journal of Biological Systems	en
dc.identifier.volume	4	en
dc.identifier.issue	3	en
dc.identifier.spage	405	en
dc.identifier.epage	423	en