Epitope mapping of the Ro/SSA60KD autoantigen reveals disease-specific antibody-binding profiles

Routsias, JG; Tzioufas, AG; SakarellosDaitsiotis, M; Sakarellos, C; Moutsopoulos, HM

dc.contributor.author	Routsias, JG	en
dc.contributor.author	Tzioufas, AG	en
dc.contributor.author	SakarellosDaitsiotis, M	en
dc.contributor.author	Sakarellos, C	en
dc.contributor.author	Moutsopoulos, HM	en
dc.date.accessioned	2014-03-01T01:45:07Z
dc.date.available	2014-03-01T01:45:07Z
dc.date.issued	1996	en
dc.identifier.issn	0014-2972	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24548
dc.subject	antiRo/SSA	en
dc.subject	epitope mapping	en
dc.subject	molecular mimicry	en
dc.subject	Sjogren's syndrome	en
dc.subject	systemic lupus erythematosus	en
dc.subject.classification	Medicine, General & Internal	en
dc.subject.classification	Medicine, Research & Experimental	en
dc.subject.other	SYSTEMIC LUPUS-ERYTHEMATOSUS	en
dc.subject.other	RO RIBONUCLEOPROTEIN	en
dc.subject.other	AUTOIMMUNE-DISEASE	en
dc.subject.other	SYNTHETIC PEPTIDES	en
dc.subject.other	ANTIGENIC REGIONS	en
dc.subject.other	SJOGRENS-SYNDROME	en
dc.subject.other	PROTEINS	en
dc.subject.other	AUTOANTIBODIES	en
dc.subject.other	PREDICTION	en
dc.subject.other	ACID	en
dc.title	Epitope mapping of the Ro/SSA60KD autoantigen reveals disease-specific antibody-binding profiles	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	Anti-Ro60KD autoantibodies are commonly found in sera from patients with primary Sjogren's syndrome (SS) and systemic lupus erythematosus (SLE). In order to identify the epitopes of this autoantigen, 22-mer, synthetic peptides overlapping by eight residues, and covering the entire sequence of the Ro60KD autoantigen were prepared. Three groups of sera were evaluated according to their autoantibody specificities. The first group consisted of monospecific anti Ro60KD sera from four patients with SLE and one with SS, the second one was composed of anti-Ro60KD + anti-La(SSB)-positive sera from four patients with SS and the third group included three normal sera and one anti Ro52KD serum. It was found that sera from SLE patients interact with a common antigenic site spanning the sequence TKYKQRNGWSHKDLLRSHLKP (169-190) of the Ro60KD protein. On the other hand, sera from SS patients recognise the ELYKEKALSVETEKLLKYLEAV (211-232) region of this autoantigen. Determination of the minimal required peptide length for optimal antibody recognition showed that the defined epitopes can be shortened to the NGWSHKDLLR (175-184) and KALSVETEKLLKYLEAV (216-332) sequences respectively. Inhibition experiments using the Ro60KD antigen and soluble peptides corresponding to the 175-184 and 216-232 segments further confirmed the specific antibody binding. These results, although only a small number of sera were used, indicate that the Ro60KD autoantigen, which is not characterized by disease specificity, contains two discrete epitopes specifically recognized from SLE and SS patient sera. Finally, the sequence similarity of the NGWSHKDLLR (175-184) epitope with some of the HLA haplotypes, associated with anti-lie response, deserves to be noted.	en
heal.publisher	BLACKWELL SCIENCE LTD	en
heal.journalName	EUROPEAN JOURNAL OF CLINICAL INVESTIGATION	en
dc.identifier.isi	ISI:A1996UV06100015	en
dc.identifier.volume	26	en
dc.identifier.issue	6	en
dc.identifier.spage	514	en
dc.identifier.epage	521	en