Production and partial characterisation of extracellular lipase from Aspergillus niger

Hatzinikolaou, DG; Macris, JB; Christakopoulos, P; Kekos, D

dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Macris, JB	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Kekos, D	en
dc.date.accessioned	2014-03-01T01:45:19Z
dc.date.available	2014-03-01T01:45:19Z
dc.date.issued	1996	en
dc.identifier.issn	0141-5492	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24570
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	GEOTRICHUM-CANDIDUM	en
dc.subject.other	MICROBIAL LIPASES	en
dc.subject.other	GROWTH-CONDITIONS	en
dc.subject.other	PURIFICATION	en
dc.title	Production and partial characterisation of extracellular lipase from Aspergillus niger	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	The production and certain kinetic characteristics of extracellular lipase from Aspergillus niger were investigated. it was possible to substantially enhance the activity of excreted lipase by optimising the interaction between carbon and nitrogen sources applying a two-parameter complete experimental design and response surface analysis. The enzyme was partially purified and a number of kinetic characteristics such as optimum pH and temperature, thermal and pH stability and K-m were determined and discussed. The elevated levels of lipase activity (40.5 U/ml) found in this work competed favourably with most of those reported for lipase hyperproducing fungi.	en
heal.publisher	CHAPMAN HALL LTD	en
heal.journalName	BIOTECHNOLOGY LETTERS	en
dc.identifier.isi	ISI:A1996UM86200013	en
dc.identifier.volume	18	en
dc.identifier.issue	5	en
dc.identifier.spage	547	en
dc.identifier.epage	552	en