Recognition of subsets of the mammalian A/B-type core heterogeneous nuclear ribonucleoprotein polypeptides by novel autoantibodies

Dangli, A; Plomaritoglou, A; Boutou, E; Vassiliadou, N; Moutsopoulos, HM; Guialis, A

dc.contributor.author	Dangli, A	en
dc.contributor.author	Plomaritoglou, A	en
dc.contributor.author	Boutou, E	en
dc.contributor.author	Vassiliadou, N	en
dc.contributor.author	Moutsopoulos, HM	en
dc.contributor.author	Guialis, A	en
dc.date.accessioned	2014-03-01T01:45:20Z
dc.date.available	2014-03-01T01:45:20Z
dc.date.issued	1996	en
dc.identifier.issn	0264-6021	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24572
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	RNA-BINDING PROTEINS	en
dc.subject.other	HNRNP PROTEIN	en
dc.subject.other	GEL-ELECTROPHORESIS	en
dc.subject.other	MESSENGER-RNA	en
dc.subject.other	PARTICLES	en
dc.subject.other	COMPLEXES	en
dc.subject.other	GENE	en
dc.subject.other	IDENTIFICATION	en
dc.subject.other	ANTIBODIES	en
dc.subject.other	PHOSPHORYLATION	en
dc.title	Recognition of subsets of the mammalian A/B-type core heterogeneous nuclear ribonucleoprotein polypeptides by novel autoantibodies	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1996	en
heal.abstract	The structurally related A/B-type core heterogeneous nuclear ribonucleoprotein (hnRNP) polypeptides of 34-39 kDa (A1, A2, B1 and B2) belong to a family of RNA-binding proteins that are major components of 40 S hnRNP complexes. By two-dimensional gel electrophoresis and peptide mapping analysis we compared each member of the A/B-type core proteins in the human and rat liver cells. This comparison revealed the unique presence in rat cells of major protein species, referred to as mBx polypeptides, that appeared as three charge isoforms at a position corresponding to the minor HeLa Bib protein spot. In addition, clear differences in the ratios of the A1 polypeptide to the A1b isoform were observed. The detection, in sera of patients with rheumatic autoimmune diseases, of two novel autoantibody specificities, one recognizing solely B2 protein and the second both the B2 and mBx polypeptides, helped to identify mBx proteins as new A/B-type hnRNP components, immunologically related to B2 protein. A common immunoreactive V8 protease peptide of approx. 17 kDa has been identified in B2 and mBx hnRNP polypeptides. mBx protein species are identified in cells of murine origin, and have a ubiquitous tissue distribution and developmental appearance.	en
heal.publisher	PORTLAND PRESS	en
heal.journalName	BIOCHEMICAL JOURNAL	en
dc.identifier.isi	ISI:A1996VZ86800009	en
dc.identifier.volume	320	en
dc.identifier.spage	761	en
dc.identifier.epage	767	en