Pseudomonas cepacia lipase localization in lecitihin and AOT w/o microemulsions. a fluorescence energy transfer study

Avramiotis, S; Stamatis, H; Kolisis, FN; Xenakis, A

dc.contributor.author	Avramiotis, S	en
dc.contributor.author	Stamatis, H	en
dc.contributor.author	Kolisis, FN	en
dc.contributor.author	Xenakis, A	en
dc.date.accessioned	2014-03-01T01:46:06Z
dc.date.available	2014-03-01T01:46:06Z
dc.date.issued	1997	en
dc.identifier.issn	0340255X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24850
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0344256216&partnerID=40&md5=fcd315d513c35a87bdcbad513aab849d	en
dc.subject	Lecithin	en
dc.subject	Lipase	en
dc.subject	Microemulsions	en
dc.subject	Reverse micelles	en
dc.title	Pseudomonas cepacia lipase localization in lecitihin and AOT w/o microemulsions. a fluorescence energy transfer study	en
heal.type	journalArticle	en
heal.publicationDate	1997	en
heal.abstract	Two water-in-oil microemulsion systems formulated with lecithin and AOT were used to solubilize lipase from Pseudomonas cepacia. The site of localization of the enzyme within the different microdomains of the dispersed phase was investigated by applying the fluorescence energy transfer technique. The nonradiative energy transfer from the tryptophan residues of lipase to an adequate acceptor molecule, such as cis-parinaric acid, was studied in both systems. The effect of the water content of the microemulsions, as well as the effect of the molar ratio of lipase to cis-parinaric acid was examined. The results showed that in both microemulsion systems lipase is preferentially localized near the surfactant membrane and that in the case of the lecithin-based systems the interface is less penetrable than the AOT one.	en
heal.journalName	Progress in Colloid and Polymer Science	en
dc.identifier.volume	105	en
dc.identifier.spage	180	en
dc.identifier.epage	183	en