An efficient and optimized purification procedure for the superoxide dismutase from Aspergillus niger . Partial characterization of the purified enzyme

Hatzinikolaou, D; Tsoukia, C; Kekos, D; Macris, B

dc.contributor.author	Hatzinikolaou, D	en
dc.contributor.author	Tsoukia, C	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, B	en
dc.date.accessioned	2014-03-01T01:46:40Z
dc.date.available	2014-03-01T01:46:40Z
dc.date.issued	1998	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/24979
dc.subject	Aspergillus Niger	en
dc.subject	Enzyme	en
dc.subject	Response Surface Methodology	en
dc.subject	Superoxide Dismutase	en
dc.subject	Molecular Weight	en
dc.subject	Specific Activity	en
dc.title	An efficient and optimized purification procedure for the superoxide dismutase from Aspergillus niger . Partial characterization of the purified enzyme	en
heal.type	journalArticle	en
heal.identifier.primary	10.1023/A:1007986510153	en
heal.identifier.secondary	http://dx.doi.org/10.1023/A:1007986510153	en
heal.publicationDate	1998	en
heal.abstract	Cu, Zn-superoxide dismutase was isolated from Aspergillus niger mycelia, harvested at the mid-logarithmic growth phase. The purification scheme aimed at the optimization of the ethanol/chloroform extraction (Tsuchihashi extraction) through response surface methodology. Upon optimum extraction conditions, it was possible to obtain electrophoretically pure enzyme preparations, by the application of one step anion exchange chromatography. The enzyme yield of this simple	en
dc.identifier.doi	10.1023/A:1007986510153	en