Factors affecting the specificity of beta-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-beta-D-glucosides

Makropoulou, M; Christakopoulos, P; Tsitsimpikou, C; Kekos, D; Kolisis, FN; Macris, BJ

dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Tsitsimpikou, C	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Kolisis, FN	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:47:35Z
dc.date.available	2014-03-01T01:47:35Z
dc.date.issued	1998	en
dc.identifier.issn	0141-8130	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/25266
dc.subject	beta-glucosidase	en
dc.subject	transglucosylation	en
dc.subject	Fusarium oxysporum	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.other	HYDROLYSIS REACTION	en
dc.subject.other	REVERSE HYDROLYSIS	en
dc.subject.other	PURIFICATION	en
dc.subject.other	ENDO-1,4-BETA-D-GLUCANASE	en
dc.title	Factors affecting the specificity of beta-glucosidase from Fusarium oxysporum in enzymatic synthesis of alkyl-beta-D-glucosides	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1998	en
heal.abstract	Fusarium oxysporum beta-glucosidase has been used to catalyze the production of alkyl-beta-D-glucosides from various disaccharides, based on the transglucosylation reaction, in the presence of primary, secondary and tertiary alcohols as glucosyl accepters. Primary alcohols were found to be the best accepters. The influence of the glucosyl donor concentration, as well as the enzyme specificity towards the cleaved glucosidic bond and the aglucone part of the donor, have also been investigated. The enzyme does not exhibit regiospecificity and seems to be unspecific towards the aglucone part. The specificity of the beta linkage has been confirmed by proton nuclear magnetic resonance (H-1 NMR) analysis. (C) 1998 Elsevier Science B.V. All rights reserved.	en
heal.publisher	ELSEVIER SCIENCE BV	en
heal.journalName	INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES	en
dc.identifier.isi	ISI:000073332900004	en
dc.identifier.volume	22	en
dc.identifier.issue	2	en
dc.identifier.spage	97	en
dc.identifier.epage	101	en