Laser excited autofluorescence for discrimination of atherosclerosis

Makropoulou, M; Drakaki, H; Anastassopoulou, N; Raptis, YS; Serafetinides, AA; Pafiti, A; Tsiligiris, B; Arapoglou, B; Demakakos, P

dc.contributor.author	Makropoulou, M	en
dc.contributor.author	Drakaki, H	en
dc.contributor.author	Anastassopoulou, N	en
dc.contributor.author	Raptis, YS	en
dc.contributor.author	Serafetinides, AA	en
dc.contributor.author	Pafiti, A	en
dc.contributor.author	Tsiligiris, B	en
dc.contributor.author	Arapoglou, B	en
dc.contributor.author	Demakakos, P	en
dc.date.accessioned	2014-03-01T01:48:36Z
dc.date.available	2014-03-01T01:48:36Z
dc.date.issued	1999	en
dc.identifier.issn	0277786X	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/25532
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-0032659191&partnerID=40&md5=1f33fc7a5be2457c9c772e0814af8bc7	en
dc.subject.other	Blood vessels	en
dc.subject.other	Calcification (biochemistry)	en
dc.subject.other	Chromophores	en
dc.subject.other	Diagnosis	en
dc.subject.other	Fluorescence	en
dc.subject.other	Laser applications	en
dc.subject.other	Lipids	en
dc.subject.other	Spectroscopic analysis	en
dc.subject.other	Tissue	en
dc.subject.other	Ultraviolet radiation	en
dc.subject.other	Laser excited autofluorescence	en
dc.subject.other	Noninvasive medical procedures	en
dc.title	Laser excited autofluorescence for discrimination of atherosclerosis	en
heal.type	journalArticle	en
heal.publicationDate	1999	en
heal.abstract	Experiments on atherosclerotic plaque diagnosis were carried out using laser induced fluorescence (LIF) spectroscopy on carotid plaque specimens. The excitation laser was a nitrogen laser, emitting pulses at a wavelength of 337 nm. Over 10 samples were examined in vitro and several spectra were obtained from each sample. Results were compared with conventional clinical techniques, such as histopathological diagnosis, which showed three areas of different composition on the pathological samples: fibrous tissue, lipid constituents and calcified plaque. An effort was made to distinguish the composition of the sample from the obtained spectra. Also, the results were compared with our previous work using longer excitation wavelengths. Spectral morphology of UV excited fluorescence reveals multi-peaks lineshapes, as a result of the superposition of different tissue chromophore signals. However, there was no observed specific wavelength where spectra corresponding to fibrous tissue, calcified tissue and lipid constituents have peaks.	en
heal.publisher	Society of Photo-Optical Instrumentation Engineers, Bellingham, WA, United States	en
heal.journalName	Proceedings of SPIE - The International Society for Optical Engineering	en
dc.identifier.volume	3564	en
dc.identifier.spage	68	en
dc.identifier.epage	75	en