Cerebrospinal fluid levels of biotin in various neurological disorders

Anagnostouli, M; Livaniou, E; Nyalala, JO; Evangelatos, G; Zournas, C; Ithakissios, DS; Papageorgiou, C

dc.contributor.author	Anagnostouli, M	en
dc.contributor.author	Livaniou, E	en
dc.contributor.author	Nyalala, JO	en
dc.contributor.author	Evangelatos, G	en
dc.contributor.author	Zournas, C	en
dc.contributor.author	Ithakissios, DS	en
dc.contributor.author	Papageorgiou, C	en
dc.date.accessioned	2014-03-01T01:48:50Z
dc.date.available	2014-03-01T01:48:50Z
dc.date.issued	1999	en
dc.identifier.issn	0001-6314	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/25614
dc.subject	biotin	en
dc.subject	cerebrospinal fluid	en
dc.subject	serum	en
dc.subject	neurological disorder	en
dc.subject.classification	Clinical Neurology	en
dc.subject.other	DIAGNOSTIC-CRITERIA	en
dc.subject.other	RADIOLIGAND ASSAY	en
dc.subject.other	DEFICIENCY	en
dc.subject.other	ALZHEIMERS	en
dc.subject.other	DISEASE	en
dc.title	Cerebrospinal fluid levels of biotin in various neurological disorders	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	1999	en
heal.abstract	Objectives - To analyse biotin concentrations in human cerebrospinal fluid (CSF) and serum from controls without evidence of nutritional or neurological disorders and patients with common neurological disorders. Patients and methods - Cerebrospinal fluid was obtained from patients by lumbar puncture, serum was prepared from freshly drawn whole blood and biotinidase in samples was inhibited before being analysed for biotin by radioligand assay. Results - Assay characteristics were within an acceptable range (intra-and interassay coefficient of variations were 8.8 and 12.0 respectively, recovery: 91-114% and sensitive, lowest standard concentration 15 ng/l). Significantly lower values for biotin were found in patients with multiple sclerosis (both CSF and serum) in comparison to the controls. Significantly reduced values for cerebrospinal fluid biotin were found in epileptics compared to controls, whereas, in serum the difference was approaching significance. No significant differences were observed in other groups of patients. Conclusion - There is a significant reduction in cerebrospinal fluid biotin in epileptics and patients with multiple sclerosis compared to controls. In epileptics this may be related to competition between biotin and anticonvulsants bearing carbamide ring for absorption. Reduction of biotin levels in patients with multiple sclerosis could be attributed to intestinal malabsorption caused by the underlying disease or a biotin-binding immunoglobulin which may be involved in multiple sclerosis pathogenesis.	en
heal.publisher	MUNKSGAARD INT PUBL LTD	en
heal.journalName	ACTA NEUROLOGICA SCANDINAVICA	en
dc.identifier.isi	ISI:000080642700010	en
dc.identifier.volume	99	en
dc.identifier.issue	6	en
dc.identifier.spage	387	en
dc.identifier.epage	392	en