Apoptosis in bladder carcinomas detected with monoclonal antibody to single-stranded DNA: Relation to cell cycle regulators and survival

Korkolopoulou, P; Konstantinidou, AE; Christodoulou, P; Patsouris, E; Thomas-Tsangli, E; Kapralos, P; Davaris, P

dc.contributor.author	Korkolopoulou, P	en
dc.contributor.author	Konstantinidou, AE	en
dc.contributor.author	Christodoulou, P	en
dc.contributor.author	Patsouris, E	en
dc.contributor.author	Thomas-Tsangli, E	en
dc.contributor.author	Kapralos, P	en
dc.contributor.author	Davaris, P	en
dc.date.accessioned	2014-03-01T01:49:46Z
dc.date.available	2014-03-01T01:49:46Z
dc.date.issued	2000	en
dc.identifier.issn	0090-4295	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/25912
dc.subject.classification	Urology & Nephrology	en
dc.subject.other	URINARY-BLADDER	en
dc.subject.other	TUMOR PROGRESSION	en
dc.subject.other	BCL-2 EXPRESSION	en
dc.subject.other	PROGNOSTIC VALUE	en
dc.subject.other	CANCER	en
dc.subject.other	P27(KIP1)	en
dc.subject.other	DEATH	en
dc.subject.other	P53	en
dc.subject.other	PROLIFERATION	en
dc.subject.other	NECROSIS	en
dc.title	Apoptosis in bladder carcinomas detected with monoclonal antibody to single-stranded DNA: Relation to cell cycle regulators and survival	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	Objectives. To investigate the incidence of baseline apoptosis in relation to p27(Kip1), p53, and p21(Cip1) expression, proliferation status, standard clinicopathologic parameters, and patient outcome. Cell cycle regulators and apoptotic cell death have been implicated in tumor aggressiveness in many human malignancies. Their interaction, however, in the prognosis of patients with transitional cell carcinoma (TCC) of the urinary bladder has not yet received intense scrutiny. Methods. Apoptotic fractions were quantified immunohistochemically by means of a novel monoclonal antibody recognizing single-stranded DNA regions in apoptotic nuclei in 103 paraffin-embedded primary TCC specimens. Proliferative activity was expressed as the percentage of Ki-67 positive cells (Ki-67 index). Tissue specimens were also stained for p27(Kip1), p53, and p21(Cip1) proteins. Patients were followed up until death (n = 30) or for an average of 40 months (median 36). Results. The apoptotic index increased with grade, T stage, nonpapillary status, proliferative activity, and p55 expression and was inversely related to p27(Kip1) and independently to p21(Cip1) expression. A negative correlation was found between p27(Kip1) expression and proliferation. The increased apoptotic index had an adverse impact on overall and disease-free survival (univariate analysis) and, along with T stage, was an independent predictor in muscle-invasive TCC. Conclusions. An increased apoptotic rate, increased proliferative activity, and decreased p21(Cip1) expression are independently interrelated in TCC. More importantly, the assessment of apoptotic potential appears to be more informative than standard prognosticators in predicting overall survival in patients with muscle-invasive TCC. UROLOGY 56: 516-520, 2000. (C) 2000, Elsevier Science Inc.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	UROLOGY	en
dc.identifier.isi	ISI:000089092100036	en
dc.identifier.volume	56	en
dc.identifier.issue	3	en
dc.identifier.spage	516	en
dc.identifier.epage	520	en