Histological assessment of apoptotic cell death in cardiomyopathies

Kavantzas, NG; Lazaris, AC; Agapitos, EV; Nanas, J; Davaris, PS

dc.contributor.author	Kavantzas, NG	en
dc.contributor.author	Lazaris, AC	en
dc.contributor.author	Agapitos, EV	en
dc.contributor.author	Nanas, J	en
dc.contributor.author	Davaris, PS	en
dc.date.accessioned	2014-03-01T01:49:54Z
dc.date.available	2014-03-01T01:49:54Z
dc.date.issued	2000	en
dc.identifier.issn	0031-3025	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/25962
dc.subject	apoptosis	en
dc.subject	TUNEL	en
dc.subject	cardiomyoparhy	en
dc.subject	arrhythmogenic dysplasia of the right ventricle	en
dc.subject.classification	Pathology	en
dc.subject.other	FRAGMENTED DNA	en
dc.subject.other	STRAND BREAKS	en
dc.subject.other	HEART	en
dc.subject.other	NECROSIS	en
dc.subject.other	FAILURE	en
dc.subject.other	INSITU	en
dc.subject.other	REPERFUSION	en
dc.subject.other	MYOCYTES	en
dc.subject.other	ABSENCE	en
dc.subject.other	HUMANS	en
dc.title	Histological assessment of apoptotic cell death in cardiomyopathies	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	Apoptosis in the myocardium is complex and often difficult to recognise. Myocyte apoptosis is scattered across the myocardial wall and is restricted to individual cells. In the present study, we describe the amount of apoptosis in 50 endomyocardial biopsies taken from 50 patients with dilated cardiomyopathy, in 14 hearts with hypertrophic cardiomyopathy and in five hearts with arrhythmogenic dysplasia of the right ventricle. As a control group, 15 endomyocardial biopsies from 15 transplanted hearts (of live patients) were used. Apoptosis was immunohistochemically determined in paraffin sections with the TUNEL method. In each specimen the TUNEL index was calculated as the percentage of TUNEL-positive nuclei among a total number of 200 counted nuclei. Cellular morphology was assessed in conjunction with TUNEL staining. The mean percentage of TUNEL-positive myocardial cells varied from 4% for dilated cardiomyopathy to 17.5% for arrhythmogenic right ventricle dysplasia and 18.5% for hypertrophic cardiomyopathy, whereas no signs of apoptotic myocardial cell death were found in normal subjects. The numbers of apoptotic cells in dilated cardiomyopathy specimens were significantly lower by comparison with both those of hypertrophic cardiomyopathy and those of arrhythmogenic right ventricular dysplasia specimens. it is evident that apoptosis constitutes a major biological phenomenon in the development of at least some heart diseases, but its role in their pathophysiology has yet to be delineated.	en
heal.publisher	CARFAX PUBLISHING	en
heal.journalName	PATHOLOGY	en
dc.identifier.isi	ISI:000088538600004	en
dc.identifier.volume	32	en
dc.identifier.issue	3	en
dc.identifier.spage	176	en
dc.identifier.epage	180	en