Purification and characterization of an extracellular alpha-L-arabinofuranosidase from Fusarium oxysporum

Christakopoulos, P; Katapodis, P; Hatzinikolaou, DG; Kekos, D; Macris, BJ

dc.contributor.author	Christakopoulos, P	en
dc.contributor.author	Katapodis, P	en
dc.contributor.author	Hatzinikolaou, DG	en
dc.contributor.author	Kekos, D	en
dc.contributor.author	Macris, BJ	en
dc.date.accessioned	2014-03-01T01:50:08Z
dc.date.available	2014-03-01T01:50:08Z
dc.date.issued	2000	en
dc.identifier.issn	0273-2289	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/26003
dc.subject	Fusarium oxysporum	en
dc.subject	alpha-L-arabinofuranosidase	en
dc.subject	purification	en
dc.subject	characterization	en
dc.subject	synergism	en
dc.subject.classification	Biochemistry & Molecular Biology	en
dc.subject.classification	Biotechnology & Applied Microbiology	en
dc.subject.other	ASPERGILLUS-AWAMORI	en
dc.subject.other	XYLANASE	en
dc.subject.other	SYSTEMS	en
dc.subject.other	WALL	en
dc.title	Purification and characterization of an extracellular alpha-L-arabinofuranosidase from Fusarium oxysporum	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2000	en
heal.abstract	An alpha-L-arabinofuranosidase from Fusarium oxysporum Fusarium oxysporum F3 was purified to homogeneity by a two-step ion exchange intercalated by a gel filtration chromatography. The enzyme had a molecular mass of 66 kDa and was optimally active at pH 6.0 and 60 degrees C. It hydrolyzed aryl alpha-L-arabinofuranosides and cleaved arabinosyl side chains from arabinoxylan and arabinan. There was a marked synergistic effect between the alpha-L-arabinofuranosidase and an endo-(1-->4)-beta-D-xylanase produced by F. oxysporum in the extensive hydrolysis of arabinoxylan.	en
heal.publisher	HUMANA PRESS INC	en
heal.journalName	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY	en
dc.identifier.isi	ISI:000088567900006	en
dc.identifier.volume	87	en
dc.identifier.issue	2	en
dc.identifier.spage	127	en
dc.identifier.epage	133	en