Quantitative RT-PCR luminometric hybridization assay with an RNA internal standard for cytokeratin-19 mRNA in peripheral blood of patients with breast cancer

Stathopoulou, A; Angelopoulou, K; Perraki, M; Georgoulias, V; Malamos, N; Lianidou, E

dc.contributor.author	Stathopoulou, A	en
dc.contributor.author	Angelopoulou, K	en
dc.contributor.author	Perraki, M	en
dc.contributor.author	Georgoulias, V	en
dc.contributor.author	Malamos, N	en
dc.contributor.author	Lianidou, E	en
dc.date.accessioned	2014-03-01T01:50:54Z
dc.date.available	2014-03-01T01:50:54Z
dc.date.issued	2001	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/26168
dc.subject	Alkaline Phosphatase	en
dc.subject	Healthy Volunteer	en
dc.subject	Internal Standard	en
dc.subject	Large Scale	en
dc.subject	Minimal Residual Disease	en
dc.subject	Peripheral Blood	en
dc.subject	Peripheral Blood Mononuclear Cell	en
dc.subject	quantitative rt-pcr	en
dc.subject	Rna Isolation	en
dc.subject	Sensitivity and Specificity	en
dc.subject	Breast Cancer	en
dc.subject	Healthy Control	en
dc.title	Quantitative RT-PCR luminometric hybridization assay with an RNA internal standard for cytokeratin-19 mRNA in peripheral blood of patients with breast cancer	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/S0009-9120(01)00276-4	en
heal.identifier.secondary	http://dx.doi.org/10.1016/S0009-9120(01)00276-4	en
heal.publicationDate	2001	en
heal.abstract	Objectives: To develop a highly sensitive quantitative RT-PCR hybridization assay for the determination of CK-19 mRNA in peripheral blood of patients with breast cancer.Patients and methods: Quantification of CK-19 mRNA was based on the coamplification of CK-19 mRNA with a recombinant CK-19 RNA internal standard (CK-19 RNA-IS) through RT-PCR. The biotinylated amplification products were immobilized on steptavidin coated wells, hybridized	en
heal.journalName	Clinical Biochemistry	en
dc.identifier.doi	10.1016/S0009-9120(01)00276-4	en