Establishment of a convenient system for the long-term culture and study of non-neoplastic human salivary gland epithelial cells

Dimitriou, ID; Kapsogeorgou, EK; Abu-Helu, RF; Moutsopoulos, HM; Manoussakis, MN

dc.contributor.author	Dimitriou, ID	en
dc.contributor.author	Kapsogeorgou, EK	en
dc.contributor.author	Abu-Helu, RF	en
dc.contributor.author	Moutsopoulos, HM	en
dc.contributor.author	Manoussakis, MN	en
dc.date.accessioned	2014-03-01T01:52:05Z
dc.date.available	2014-03-01T01:52:05Z
dc.date.issued	2002	en
dc.identifier.issn	0909-8836	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/26551
dc.subject	epithelial cells	en
dc.subject	salivary gland	en
dc.subject	culture	en
dc.subject	methods	en
dc.subject	Sjogren's syndrome	en
dc.subject.classification	Dentistry, Oral Surgery & Medicine	en
dc.subject.other	MESSENGER-RNA EXPRESSION	en
dc.subject.other	HLA-DR EXPRESSION	en
dc.subject.other	SJOGRENS-SYNDROME	en
dc.subject.other	AUTOIMMUNE EPITHELITIS	en
dc.subject.other	DUCT CELLS	en
dc.subject.other	ANTIGEN	en
dc.subject.other	LINE	en
dc.subject.other	DIFFERENTIATION	en
dc.subject.other	PHENOTYPE	en
dc.subject.other	MOLECULES	en
dc.title	Establishment of a convenient system for the long-term culture and study of non-neoplastic human salivary gland epithelial cells	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	Epithelial cells appear to play an important role in the initiation and maintenance of autoimmune lesions in the salivary glands of patients with Sjogren's syndrome. Therefore, the detailed study of immunological function of salivary gland epithelial cells (SGEC) may provide useful information for the understanding of Sjogren's syndrome pathogenesis. In this report we aimed to formulate a protocol for the establishment of human non-neoplastic SGEC lines as a tool for the study of the physiology and pathophysiology of these cells. pointing towards a practical approach, we sought to establish SGEC lines from quite a limited amount of biopsy tissue obtained during the diagnostic evaluation of patients. Herein, the favorable conditions for the long-term maintenance of human non-neoplastic SGEC lines are presented and involve the successive application of a serum-containing and a serum-free culture medium, supplemented with essential epithelial growth factors. This protocol has been found reliable and convenient, as attested by the reproducible establishment of non-neoplastic SGEC lines. The analysis of SGEC phenotypic features, as well as a coculture system for the study of interactions between epithelial cells and lymphocytes, are also described. Such techniques may provide valuable means for the functional and molecular investigation of human SGEC and particularly for the study of Sjogren's syndrome and other disorders of glandular epithelia.	en
heal.publisher	BLACKWELL MUNKSGAARD	en
heal.journalName	EUROPEAN JOURNAL OF ORAL SCIENCES	en
dc.identifier.isi	ISI:000173967600005	en
dc.identifier.volume	110	en
dc.identifier.issue	1	en
dc.identifier.spage	21	en
dc.identifier.epage	30	en