Expression of the vascular endothelial growth factor receptor-2/Fik-1 in breast carcinomas: Correlation with proliferation

Nakopoulou, L; Stefanaki, K; Panayotopoulou, E; Giannopoulou, I; Athanassiadou, P; Gakiopoulou-Givalou, H; Louvrou, A

dc.contributor.author	Nakopoulou, L	en
dc.contributor.author	Stefanaki, K	en
dc.contributor.author	Panayotopoulou, E	en
dc.contributor.author	Giannopoulou, I	en
dc.contributor.author	Athanassiadou, P	en
dc.contributor.author	Gakiopoulou-Givalou, H	en
dc.contributor.author	Louvrou, A	en
dc.date.accessioned	2014-03-01T01:52:05Z
dc.date.available	2014-03-01T01:52:05Z
dc.date.issued	2002	en
dc.identifier.issn	0046-8177	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/26553
dc.subject	fetal liver kinase (Flk-1)/KDR	en
dc.subject	breast cancer	en
dc.subject	proliferation	en
dc.subject.classification	Pathology	en
dc.subject.other	TUMOR ANGIOGENESIS	en
dc.subject.other	TISSUE INHIBITOR	en
dc.subject.other	FACTOR VEGF	en
dc.subject.other	IN-VIVO	en
dc.subject.other	MELANOMA-CELLS	en
dc.subject.other	CANCER	en
dc.subject.other	RECEPTORS	en
dc.subject.other	FLK-1	en
dc.subject.other	VASCULOGENESIS	en
dc.subject.other	ANTIBODY	en
dc.title	Expression of the vascular endothelial growth factor receptor-2/Fik-1 in breast carcinomas: Correlation with proliferation	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2002	en
heal.abstract	Vascular endothelial growth factor (VEGF) and its receptor Flk-1/KDR play an important role in vascular permeability and tumor angiogenesis. Prompted by the hypothesis that VEGF/Flk-1 system may have regulatory roles in breast carcinogenesis, we investigated the expression of Flk-1 in 141 invasive breast carcinomas in correlation with clinical and immunohistochemical prognostic parameters, including proliferation indices like Ki-67 and Topoisomerase Ha (Topo-Ha). The immunohistochemical avidin-biotin-peroxidase method was performed on paraffin sections for the detection of Flk-1, p53, Bcl-2, c-erbB-2, M-67, Topo-Ha, ER, and PR. Flk-1 was detected in 91 of 141 (64.5%) of invasive breast carcinomas showing a widespread cytoplasmic expression in most of the neoplastic cells. Flk-1 expression was correlated with the menopausal status (P = 0.051) of the patient and the nuclear grade of the invasive breast carcinoma (P = 0.003), but demonstrated no correlation with histologic grade, stage, and patient survival. It is interesting that Flk-1 expression demonstrated a significant correlation with 2 well-established proliferation indices, Ki-67 (P = 0.037) and topo-IIalpha (P = 0.009), whereas there was no correlation with the expression of ER, PR, p53, Bcl-2, and c-erbB-2. Moreover, Flk-1 expression showed an inverse correlation with TIMP-I mRNA localization in intratumoral stromal cells (P = 0.013). In conclusion, the significant correlation of Flk-1 expression in invasive breast carcinomas with proliferation indices like Ki-67 and topo-IIa suggests that VEGF may exert a growth factor activity on mammary cancer cells through its receptor Flk-1. On the other hand, the inverse correlation of Flk-1 with TIMP-1 mRNA in intratumoral stromal cells supports the notion that TIMP-1 may have an inhibitory role on angiogenesis. Copyright 2002, Elsevier Science (USA). All rights reserved.	en
heal.publisher	W B SAUNDERS CO	en
heal.journalName	HUMAN PATHOLOGY	en
dc.identifier.isi	ISI:000178282800002	en
dc.identifier.volume	33	en
dc.identifier.issue	9	en
dc.identifier.spage	863	en
dc.identifier.epage	870	en