Soluble fas concentrations in the follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: Association with oocyte maturity, fertilization, and embryo quality

Malamitsi-Puchner, A; Sarandakou, A; Baka, S; Vrachnis, N; Kouskouni, E; Hassiakos, D

dc.contributor.author	Malamitsi-Puchner, A	en
dc.contributor.author	Sarandakou, A	en
dc.contributor.author	Baka, S	en
dc.contributor.author	Vrachnis, N	en
dc.contributor.author	Kouskouni, E	en
dc.contributor.author	Hassiakos, D	en
dc.date.accessioned	2014-03-01T01:54:04Z
dc.date.available	2014-03-01T01:54:04Z
dc.date.issued	2004	en
dc.identifier.issn	1071-5576	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/27175
dc.subject	in vitro fertilization	en
dc.subject	soluble Fas	en
dc.subject	oocyte maturity	en
dc.subject	fertilization	en
dc.subject	embryo quality	en
dc.subject.classification	Obstetrics & Gynecology	en
dc.subject.other	INTRACYTOPLASMIC SPERM INJECTION	en
dc.subject.other	CELL-DEATH	en
dc.subject.other	CORRESPONDING OOCYTE	en
dc.subject.other	MATURATION STAGE	en
dc.subject.other	APOPTOSIS	en
dc.subject.other	GRANULOSA	en
dc.subject.other	CLEAVAGE	en
dc.title	Soluble fas concentrations in the follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization: Association with oocyte maturity, fertilization, and embryo quality	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2004	en
heal.abstract	OBJECTIVE: Because soluble Fas (sFas) inhibits Fas-mediated apoptosis by preventing death signal transduction, we determined sFas concentrations in the follicular fluid (FF) and oocyte-cumulus complex culture medium (CM) from women undergoing in vitro fertilization (IVF) in order to associate its concentrations with oocyte maturity, fertilization, and embryo quality. METHODS: We studied 82 follicles from 11 healthy women (mean age, 35.4 +/- 3.8 years) using a long protocol for IVF treatment. Individual FF and matched CM samples were immediately centrifuged at 4C and sFas concentrations were determined by sandwich enzyme-linked immunosorbent assays. RESULTS: sFas concentrations were significantly higher in FF than in CM (P < .0001) and when oocytcs were mature rather than immature (P < .002). Of 70 mature and 12 immature oocytes, 56 (80%) and two (16.6%), respectively, were fertilized. sFas concentrations in CM were significantly lower when mature oocytes were fertilized versus nonfertilized (P < .005). sFas concentrations in FF and CM were significantly related in an inverse manner to embryo quality (P = .004 and P = .0002, respectively). CONCLUSION: FF and CM from women undergoing IVF contain sFas. The latter has anti-apoptotic properties and levels are higher: in FF when oocytes are mature and in CM when oocytes are nonfertilized. Furthermore, FF and CM sFas concentrations are negatively correlated with embryo quality. Copyright (C) 2004 by the Society for Gynecologic Investigation.	en
heal.publisher	ELSEVIER SCIENCE INC	en
heal.journalName	JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION	en
dc.identifier.isi	ISI:000225814900011	en
dc.identifier.volume	11	en
dc.identifier.issue	8	en
dc.identifier.spage	566	en
dc.identifier.epage	569	en