Cross-recognition between histones and La/SSB may account for anti-DNA reactivity in SLE patients

Touloupi, E; Routsias, JG; Tzioufas, AG

dc.contributor.author	Touloupi, E	en
dc.contributor.author	Routsias, JG	en
dc.contributor.author	Tzioufas, AG	en
dc.date.accessioned	2014-03-01T01:54:35Z
dc.date.available	2014-03-01T01:54:35Z
dc.date.issued	2005	en
dc.identifier.issn	0009-9104	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/27438
dc.subject	anti-La/ SSB	en
dc.subject	histones	en
dc.subject	Sjogren's syndrome	en
dc.subject	systemic lupus erythematosus	en
dc.subject	B-cell epitopes	en
dc.subject.classification	Immunology	en
dc.subject.other	SYSTEMIC-LUPUS-ERYTHEMATOSUS	en
dc.subject.other	PRIMARY SJOGRENS-SYNDROME	en
dc.subject.other	CRITHIDIA-LUCILIAE	en
dc.subject.other	AUTOANTIBODIES	en
dc.subject.other	SPECIFICITY	en
dc.subject.other	ANTIBODIES	en
dc.subject.other	CLASSIFICATION	en
dc.subject.other	TRANSCRIPTS	en
dc.subject.other	COMPONENTS	en
dc.subject.other	CRITERIA	en
dc.title	Cross-recognition between histones and La/SSB may account for anti-DNA reactivity in SLE patients	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2005	en
heal.abstract	Antibodies to La/SSB are detected in sera of patients with primary Sjogren's syndrome (pSS) and systemic lupus erythematosus (SLE). The vast majority of anti-La/SSB positive sera contain antibodies directed towards a linear B-cell epitope of La/SSB spanning the sequence 349-364aa (pep349-364). The aim of this study was to evaluate the fluctuation of antibody levels to major B-cell epitopes of La/SSB over time and investigate for their possible crossreactions. Sequential sera from 15 SLE and 15 pSS patients, followed from 3 to 10 years were obtained. All patients with SLE were positive for anti-Ro/SSA, anti-La/SSB and anti-dsDNA antibodies and patients with pSS were positive for anti-Ro/SSA and anti-La/SSB antibodies. Sera from 30 patients with SLE without anti-La/SSB antibodies and 30 healthy individuals served as disease and negative control respectivelly. All sera tested for the presence of anti-pep349-364 antibodies, using a specific ELISA. Specific anti-pep349-364 IgG was purified from sera of SLE patients and evaluated for cross reactivity against dsDNA and histones. In all SLE sera the levels of anti-pep349-364 antibodies varied in time and fluctuated in parallel with anti dsDNA antibodies. Anti-pep349-364 IgG purified from 7 SLE patients. Five out of 7 were found to react with calf thymus DNA in ELISA. All purified (7/7) anti-pep349-364 IgG preparations reacted with histone H1 and failed to produce a positive immunofluorescence pattern in Crithidia luciliae anti-dsDNA assay which lacks histones. Competative inhibition experiments demonstrated that histone H1 could inhibit completely the binding of anti-pep349-364 IgG to pep349-364 while pep349-364 inhibited by 70% the binding of anti-pep349-364 IgG to histone H1. These findings indicate that a subgroup of SLE patients possess cross-reacting anti-histone H1 antibodies and anti-pep349-364 antibodies, which can be faulty considered as anti-dsDNA reactivity in regular ELISA techniques.	en
heal.publisher	BLACKWELL PUBLISHING	en
heal.journalName	CLINICAL AND EXPERIMENTAL IMMUNOLOGY	en
dc.identifier.isi	ISI:000231824900024	en
dc.identifier.volume	142	en
dc.identifier.issue	1	en
dc.identifier.spage	172	en
dc.identifier.epage	179	en