The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits

Andreadou, I; Iliodromitis, EK; Mikros, E; Constantinou, M; Agalias, A; Magiatis, P; Skaltsounis, AL; Kamber, E; Tsantili-Kakoulidou, A; Kremastinos, DT

dc.contributor.author	Andreadou, I	en
dc.contributor.author	Iliodromitis, EK	en
dc.contributor.author	Mikros, E	en
dc.contributor.author	Constantinou, M	en
dc.contributor.author	Agalias, A	en
dc.contributor.author	Magiatis, P	en
dc.contributor.author	Skaltsounis, AL	en
dc.contributor.author	Kamber, E	en
dc.contributor.author	Tsantili-Kakoulidou, A	en
dc.contributor.author	Kremastinos, DT	en
dc.date.accessioned	2014-03-01T01:55:43Z
dc.date.available	2014-03-01T01:55:43Z
dc.date.issued	2006	en
dc.identifier.issn	0022-3166	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/27818
dc.subject.classification	Nutrition & Dietetics	en
dc.subject.other	RADICAL-SCAVENGING PROPERTIES	en
dc.subject.other	MYOCARDIAL INFARCT SIZE	en
dc.subject.other	CHOLESTEROL-RICH DIET	en
dc.subject.other	NITRIC-OXIDE	en
dc.subject.other	LIPOPROTEIN OXIDATION	en
dc.subject.other	MAGNETIC-RESONANCE	en
dc.subject.other	MEDITERRANEAN DIET	en
dc.subject.other	IN-VITRO	en
dc.subject.other	OIL	en
dc.subject.other	HEART	en
dc.title	The olive constituent oleuropein exhibits anti-ischemic, antioxidative, and hypolipidemic effects in anesthetized rabbits	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2006	en
heal.abstract	The goal of this study was to evaluate the efficacy of the antioxidant olive constituent, oleuropein, on infarct size, oxidative damage, and the metabolic profile in rabbits subjected to ischemia. Oleuropein, 10 or 20 mg/(kg(.)d), was administered to 8 groups that consumed a normal or hypercholesterolemic diet for 6 wk or only the higher dose for 3 wk. Circulating levels of malondialdehyde, protein carbonyl, nitrite+nitrate, cholesterol, triglycerides, SOD activity, and the metabolic profile were measured using H-1 NMR spectra. In rabbits that consumed the normal diet, the infarct size (percentage of infarct to risk areas) was reduced by the administration of 10 mg oleuropein/(kg(.)d) (16.1 +/- 2.9%) or 20 mg oleuropein/(kg(.)d) for 3 wk (21.7 +/- 2.2%) or for 6 wk (24.3 +/- 1.3%) compared with the control group (48.05 +/- 2.0%, P < 0.05). Only the higher dose of 20 mg/(kg(.)d) reduced the infarct size in hypercholesterolemic rabbits (34.7 +/- 4.4% for 6 wk and 34.8 +/- 6.1 % for 3 wk) compared with the cholesterol-fed control group (52.8 +/- 2.4%, P < 0.05). Oleuropein decreased the plasma lipid peroxidation product and protein carbonyl concentrations compared with the control groups, in which these factors increased relative to baseline due to ischemia and reperfusion. Furthermore, in rabbits administered oleuropein, RBC superoxide dismutase activity did not change during ischemia and reperfusion. This activity was significantly higher than in both control groups in which it was reduced by ischemia and reperfusion compared with baseline. Treatment for 6 wk with both doses of oleuropein reduced total cholesterol and triglyceride concentrations. H-1 NMR spectra revealed a different profile of glycolysis metabolites in the oleuropein-treated groups compared with the controls. Oleuropein, for 3 or 6 wk, reduced the infarct size, conferred strong antioxidant protection and reduced the circulating lipids. This is the first experimental study in vivo that suggests the possibility of using an olive constituent in the treatment, of ischemia.	en
heal.publisher	AMER SOCIETY NUTRITIONAL SCIENCE	en
heal.journalName	JOURNAL OF NUTRITION	en
dc.identifier.isi	ISI:000239366300016	en
dc.identifier.volume	136	en
dc.identifier.issue	8	en
dc.identifier.spage	2213	en
dc.identifier.epage	2219	en