Coating of human mesenchymal cells in 3D culture with bioinorganic nanoparticles promotes osteoblastic differentiation and gene transfection

Gonzalez-McQuire, R; Green, DW; Partridge, KA; Oreffo, ROC; Mann, S; Davis, SA

dc.contributor.author	Gonzalez-McQuire, R	en
dc.contributor.author	Green, DW	en
dc.contributor.author	Partridge, KA	en
dc.contributor.author	Oreffo, ROC	en
dc.contributor.author	Mann, S	en
dc.contributor.author	Davis, SA	en
dc.date.accessioned	2014-03-01T01:56:18Z
dc.date.available	2014-03-01T01:56:18Z
dc.date.issued	2007	en
dc.identifier.issn	09359648	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28041
dc.subject.other	Amino acids	en
dc.subject.other	Bone	en
dc.subject.other	Calcium phosphate	en
dc.subject.other	Coatings	en
dc.subject.other	Gene expression	en
dc.subject.other	Nanoparticles	en
dc.subject.other	Osteoblasts	en
dc.subject.other	Three dimensional	en
dc.subject.other	Bone matrix production	en
dc.subject.other	Differentiation	en
dc.subject.other	Surface coatings	en
dc.subject.other	Transfection	en
dc.subject.other	Cell culture	en
dc.title	Coating of human mesenchymal cells in 3D culture with bioinorganic nanoparticles promotes osteoblastic differentiation and gene transfection	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/adma.200602770	en
heal.identifier.secondary	http://dx.doi.org/10.1002/adma.200602770	en
heal.publicationDate	2007	en
heal.abstract	Coating of human mesenchymal stem cells (MSC) in 3D culture with bioinorganic nanoparticles helps in promoting osteoblastic differentiation and gene transfection. Modifying human MSCs directly in aqueous suspension with surface coatings of amino acid-functionalized calcium phosphate nanoparticles offers new opportunities for preparing a range of osteoinductive hybrid 'living' biomaterials. This 3D coating strategy produces viable cell/hydroxyapatite (HAP) conjugates and plasmid DNA transfected constructs, by simple and efficient methods. The bioinorganic coating is sufficient to stimulate MSC differentiation and bone matrix production in the absence of osteogenic media. Furthermore, the incorporation of specific biomolecular stimuli in coated cell aggregates enhances gene expression compared to 2D methods involving adherent cells, and that this protocol is also applicable to primary human cells.	en
heal.journalName	Advanced Materials	en
dc.identifier.doi	10.1002/adma.200602770	en
dc.identifier.volume	19	en
dc.identifier.issue	17	en
dc.identifier.spage	2236	en
dc.identifier.epage	2240	en