GATA-1 transcription factor is up-regulated in bone marrow hematopoietic progenitor CD34(+) and erythroid CD71(+) cells in myelodysplastic syndromes

Maratheftis, CI; Bolaraki, PE; Voulgarelis, M

dc.contributor.author	Maratheftis, CI	en
dc.contributor.author	Bolaraki, PE	en
dc.contributor.author	Voulgarelis, M	en
dc.date.accessioned	2014-03-01T01:56:29Z
dc.date.available	2014-03-01T01:56:29Z
dc.date.issued	2007	en
dc.identifier.issn	0361-8609	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28119
dc.subject.classification	Hematology	en
dc.subject.other	INHERITED MUTATION	en
dc.subject.other	ACQUIRED MUTATIONS	en
dc.subject.other	DOWNS-SYNDROME	en
dc.subject.other	EXPRESSION	en
dc.subject.other	GENE	en
dc.subject.other	DIFFERENTIATION	en
dc.subject.other	LEUKEMIA	en
dc.subject.other	THROMBOCYTOPENIA	en
dc.subject.other	DISORDER	en
dc.title	GATA-1 transcription factor is up-regulated in bone marrow hematopoietic progenitor CD34(+) and erythroid CD71(+) cells in myelodysplastic syndromes	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2007	en
heal.abstract	GATA-1 is a transcription factor governing the production of erythroid and megalkaryocytic cells. Unobstructed GATA-1 expression in early progenitor cells commits them to the myeloid lineage, channeling its differentiation towards erythrocytes and megakaryocytes. Myelodysplastic Syndromes (MDS) are clonal disorders of the hematopoietic stem cell frequently presenting dysplasia in erythroid and/or megalkaryocytic lineage. We reasoned that measurement of GATA-1 expression levels in hematopoietic progenitor CD34(+) and the committed erythroid CD71(+) cells, from various MDS subcategories, could demonstrate GATA-1 involvement in the pathogenesis of the syndrome. In this study, MDS patients displayed significantly elevated GATA-1 mRNA expression, in bond marrow mononuclear cells (BMMCs), progenitor CD34(+) and erythroid CD71(+) cells in contrast to the control population (P < 0.001). Additionally, GATA-1 mRNA expression in MIDS CD71(+) cells was positively correlated with their apoptotic levels (p = 0.58, P = 0.03). Furthermore, GATA-1 expression levels were found to correlate with the disease progression. MDS patients in high/INT-2 IPSS risk group expressed significantly higher GATA-1 mRNA levels, in both CD34(+) and CD71(+) cells, as opposed to low/INT-1 patients (P < 0.001). Moreover, the former displayed increased apoptosis in the CD71(+) cells and significantly reduced neutrophil and platelet numbers and hemoglobin levels compared with the latter. We conclude that MDS patients display an increase of GATA-1 mRNA expression in BM cells, with high/INT-2 patients showing significantly higher levels. The higher level of GATA-1 mRNA in erythroid cells was positively correlated with their degree of apoptosis. These findings suggest that the up-regulation of GATA-1 may be responsible for the peripheral cytopenias in MDS.	en
heal.publisher	WILEY-LISS	en
heal.journalName	AMERICAN JOURNAL OF HEMATOLOGY	en
dc.identifier.isi	ISI:000249794500006	en
dc.identifier.volume	82	en
dc.identifier.issue	10	en
dc.identifier.spage	887	en
dc.identifier.epage	892	en