Evaluation of immunohistochemical markers of germ cells' proliferation in the developing rat testis: A comparative study

Angelopoulou, R; Balla, M; Lavranos, G; Chalikias, M; Kitsos, C; Baka, S; Kittas, C

dc.contributor.author	Angelopoulou, R	en
dc.contributor.author	Balla, M	en
dc.contributor.author	Lavranos, G	en
dc.contributor.author	Chalikias, M	en
dc.contributor.author	Kitsos, C	en
dc.contributor.author	Baka, S	en
dc.contributor.author	Kittas, C	en
dc.date.accessioned	2014-03-01T01:57:07Z
dc.date.available	2014-03-01T01:57:07Z
dc.date.issued	2008	en
dc.identifier.issn	00408166	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28354
dc.subject	Cell proliferation	en
dc.subject	Germ cells	en
dc.subject	Ki-67	en
dc.subject	Labeling index	en
dc.subject	PCNA	en
dc.subject	Testis	en
dc.subject.other	antinuclear antibody	en
dc.subject.other	cycline	en
dc.subject.other	Ki 67 antigen	en
dc.subject.other	animal tissue	en
dc.subject.other	article	en
dc.subject.other	birth	en
dc.subject.other	cell count	en
dc.subject.other	cell density	en
dc.subject.other	cell proliferation	en
dc.subject.other	comparative study	en
dc.subject.other	computer program	en
dc.subject.other	controlled study	en
dc.subject.other	DNA repair	en
dc.subject.other	embryo	en
dc.subject.other	fetus	en
dc.subject.other	germ cell	en
dc.subject.other	immunohistochemistry	en
dc.subject.other	male	en
dc.subject.other	mitosis inhibition	en
dc.subject.other	newborn	en
dc.subject.other	nonhuman	en
dc.subject.other	perinatal period	en
dc.subject.other	prenatal period	en
dc.subject.other	protein function	en
dc.subject.other	rat	en
dc.subject.other	testis development	en
dc.subject.other	Wistar rat	en
dc.subject.other	Animals	en
dc.subject.other	Antigens, Nuclear	en
dc.subject.other	Biological Markers	en
dc.subject.other	Cell Proliferation	en
dc.subject.other	Female	en
dc.subject.other	Immunohistochemistry	en
dc.subject.other	Male	en
dc.subject.other	Rats	en
dc.subject.other	Rats, Wistar	en
dc.subject.other	Spermatozoa	en
dc.subject.other	Testis	en
dc.subject.other	Rattus	en
dc.subject.other	Rattus norvegicus	en
dc.title	Evaluation of immunohistochemical markers of germ cells' proliferation in the developing rat testis: A comparative study	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.tice.2007.09.004	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.tice.2007.09.004	en
heal.publicationDate	2008	en
heal.abstract	Germ cells' proliferation during testicular organogenesis in Wistar rat embryos and neonates [14.5, 18.5, 20.5 days post conception (dpc), birth (day 0), 1, 3, 5, 7 days post partum (dpp)] was evaluated via immunohistochemistry, using the PCNA and Ki-67 nuclear antibodies. Estimation of the reactive/total cell ratio, per visual field [labeIing index (LI)] was achieved using the Image Pro Plus Software. Immunostaining of the fetal testis, with both antibodies, revealed increasing germ cells' numbers between 14.5 dpc and birth. From birth onwards, a sharp decline of germ cells' population was observed in the first 3 days of postnatal life. Then, a transient increase of the LI, between 3 and 5 dpp, was noted. Afterwards, proliferation of germ cells ceased. These results indicate that, during fetal and neonatal life, two peaks of proliferative activity of germ cells are noticed. Following estimation of the LI for both PCNA and Ki-67, a prominent labeling for the first antibody was observed throughout the examined period. Ki-67 staining follows a similar pattern, showing, however, significant fluctuation in the obtained values, in comparison to PCNA. The significant differences observed don't seem to be simply a result of the different half lives of the two markers, but rather a consequence of additional underlying cellular activity associated with PCNA, such as DNA repair. © 2007 Elsevier Ltd. All rights reserved.	en
heal.journalName	Tissue and Cell	en
dc.identifier.doi	10.1016/j.tice.2007.09.004	en
dc.identifier.volume	40	en
dc.identifier.issue	1	en
dc.identifier.spage	43	en
dc.identifier.epage	50	en