Sertoli cell proliferation in the fetal and neonatal rat testis: A continuous phenomenon?

Angelopoulou, R; Balla, M; Lavranos, G; Chalikias, M; Kitsos, C; Baka, S; Kittas, C

dc.contributor.author	Angelopoulou, R	en
dc.contributor.author	Balla, M	en
dc.contributor.author	Lavranos, G	en
dc.contributor.author	Chalikias, M	en
dc.contributor.author	Kitsos, C	en
dc.contributor.author	Baka, S	en
dc.contributor.author	Kittas, C	en
dc.date.accessioned	2014-03-01T01:57:12Z
dc.date.available	2014-03-01T01:57:12Z
dc.date.issued	2008	en
dc.identifier.issn	00651281	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28382
dc.subject	Cell proliferation	en
dc.subject	Ki-67	en
dc.subject	Labeling index	en
dc.subject	PCNA	en
dc.subject	Sertoli cells	en
dc.subject	Testis	en
dc.subject.other	cycline	en
dc.subject.other	Ki 67 antigen	en
dc.subject.other	animal cell	en
dc.subject.other	animal experiment	en
dc.subject.other	animal tissue	en
dc.subject.other	antibody labeling	en
dc.subject.other	article	en
dc.subject.other	cell count	en
dc.subject.other	cell proliferation	en
dc.subject.other	controlled study	en
dc.subject.other	embryo	en
dc.subject.other	female	en
dc.subject.other	immunoreactivity	en
dc.subject.other	in vivo study	en
dc.subject.other	male	en
dc.subject.other	mitosis rate	en
dc.subject.other	nonhuman	en
dc.subject.other	rat	en
dc.subject.other	Sertoli cell	en
dc.subject.other	testis	en
dc.subject.other	Animals	en
dc.subject.other	Animals, Newborn	en
dc.subject.other	Cell Proliferation	en
dc.subject.other	Female	en
dc.subject.other	Fetus	en
dc.subject.other	Immunohistochemistry	en
dc.subject.other	Ki-67 Antigen	en
dc.subject.other	Male	en
dc.subject.other	Proliferating Cell Nuclear Antigen	en
dc.subject.other	Rats	en
dc.subject.other	Rats, Wistar	en
dc.subject.other	Sertoli Cells	en
dc.subject.other	Testis	en
dc.subject.other	Rattus	en
dc.subject.other	Rattus norvegicus	en
dc.title	Sertoli cell proliferation in the fetal and neonatal rat testis: A continuous phenomenon?	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.acthis.2007.10.009	en
heal.identifier.secondary	http://dx.doi.org/10.1016/j.acthis.2007.10.009	en
heal.publicationDate	2008	en
heal.abstract	Sertoli cell population kinetics, as evidenced by semi-quantitative immunolabeling for proliferating cell nuclear antigen (PCNA) and Ki-67, in developing Wistar rat male gonads of embryos and neonates [14.5 days post conception (dpc) - 7 days post partum (dpp)], was investigated. Throughout the examined period a gradual increase of immunolabeled Sertoli cell number, associated with intense mitotic activity, was observed. PCNA labeling index of Sertoli cells increased from 66.67 (at 14.5 dpc) to 89.74 (at 18.5 dpc) and then dropped to 75.24 (at 20.5 dpc). At birth, the percentage of PCNA immunoreactive Sertoli cells reached 98.70% and remained high thereafter, attaining a peak value of 99.90% at 7 dpp. The percentage of Ki-67 immunoreactive Sertoli cells in the fetal testis increased from E14.5 (43.95%) to E20.5 (77.40%). The proliferation rate did not alter considerably in the neonatal testis until 5 dpp. At this point, a significant increase of the Ki-67 labeling index was observed and a peak value of 95.76% was reached at 7 dpp. The pattern of Sertoli cell proliferation with age and the establishment of the final Sertoli cell number in vivo established in the present study was compared to the results from earlier investigations reported in the literature and the observed fluctuation of dividing cell numbers, associated with immunolabeling results throughout the examined period, complements and extends existing data. An appraisal of the timing of Sertoli cell proliferation in other species, namely mouse and man, is presented. The current investigation may be useful in evaluating the potential influence of factors interfering with normal mitotic activity of Sertoli cells, including cell selection mechanisms, such as apoptosis, senescence, DNA repair and hormonal/paracrine growth modulation. © 2007 Elsevier GmbH. All rights reserved.	en
heal.journalName	Acta Histochemica	en
dc.identifier.doi	10.1016/j.acthis.2007.10.009	en
dc.identifier.volume	110	en
dc.identifier.issue	4	en
dc.identifier.spage	341	en
dc.identifier.epage	347	en