Comparative qualitative and quantitative analysis of scleroderma (systemic sclerosis) serologic immunoassays

Picha, L; Pakas, I; Guialis, A; Moutsopoulos, HM; Vlachoyiannopoulos, PG

dc.contributor.author	Picha, L	en
dc.contributor.author	Pakas, I	en
dc.contributor.author	Guialis, A	en
dc.contributor.author	Moutsopoulos, HM	en
dc.contributor.author	Vlachoyiannopoulos, PG	en
dc.date.accessioned	2014-03-01T01:57:20Z
dc.date.available	2014-03-01T01:57:20Z
dc.date.issued	2008	en
dc.identifier.issn	0896-8411	en
dc.identifier.uri	https://dspace.lib.ntua.gr/xmlui/handle/123456789/28403
dc.subject	Antigenic source	en
dc.subject	Autoantibodies	en
dc.subject	Immunoblot	en
dc.subject	Systemic sclerosis (scleroderma)	en
dc.subject.classification	Immunology	en
dc.subject.other	LINKED-IMMUNOSORBENT-ASSAY	en
dc.subject.other	TOPOISOMERASE-I SCL-70	en
dc.subject.other	ANTICENTROMERE ANTIBODIES	en
dc.subject.other	ANTIHISTONE ANTIBODIES	en
dc.subject.other	CLINICAL ASSOCIATIONS	en
dc.subject.other	LUPUS-ERYTHEMATOSUS	en
dc.subject.other	RIBONUCLEOPROTEIN PARTICLE	en
dc.subject.other	PULMONARY-HYPERTENSION	en
dc.subject.other	POLYMYOSITIS OVERLAP	en
dc.subject.other	NUCLEOLAR ANTIBODIES	en
dc.title	Comparative qualitative and quantitative analysis of scleroderma (systemic sclerosis) serologic immunoassays	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2008	en
heal.abstract	The heterogeneity of autoantibody specificities occurring in sera from patients with systemic sclerosis (SSc) raised the necessity of developing various methodologies for their detection. A cohort of 150 SSc patients were selected and tested by Indirect Immunofluorescence (IIF), Counterimmunoelectrophoresis (CIE), Immunoblot (IB) using various extracts as antigenic source and RNA precipitation. By preparing a nuclear (IB-nuclear) and a metaphase chromosomal-enriched extract (IB-MC-pellet) from HeLa cells as well as a nucleolar (IB-nucleolar) and a histone (IB-histone) extract from rat liver nuclei, we assessed their sensitivity and specificity for anti-Topo 1, anti-U3RNP, anti-H-1, anti-snRNPs antibodies and ACA. IB-nuclear revealed the highest frequency of anti-Topo I antibodies, while CIE, IB-nucleolar and IB-MC-pellet, when compared to IB-nuclear showed a sensitivity of 89%, 87% and 95%, respectively. IB-MC-pellet was unique for ACA recognition, while IB-nucleolar and IB-MC-pellet showed excellent sensitivity for anti-U3RNP and anti-H, antibody detection. We conclude that IB-nuclear is a highly sensitive system for anti Topo I antibodies determination, but CIE reveals a good sensitivity to be used as a first screening test. IB-nucleolar or IB-MC-pellet are important techniques to detect the variety of antibodies to nucleolus and chromatin-related constituents. A novel specificity against a 28 kD nucleolar protein, non-associated with RNAs is also presented. (C) 2008 Elsevier Ltd. All rights reserved.	en
heal.publisher	ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD	en
heal.journalName	JOURNAL OF AUTOIMMUNITY	en
dc.identifier.isi	ISI:000259880700012	en
dc.identifier.volume	31	en
dc.identifier.issue	2	en
dc.identifier.spage	166	en
dc.identifier.epage	174	en